USDA-ARS, Fort Keogh Livestock and Range Research Laboratory, Miles City, MT 59301, USA.
J Anim Sci. 2010 Mar;88(3):943-9. doi: 10.2527/jas.2009-2087. Epub 2009 Nov 20.
The objective of this research was to determine effects of a single injection of the PG synthesis inhibitor flunixin meglumine (FM; 1.1 mg/kg of BW, intramuscularly) approximately 13 d (range 10 to 15 d) after AI on pregnancy establishment. Three experiments were conducted using estrus-synchronized heifers and cows. Technicians and AI sires were equally represented across treatments within locations and experiments. Bulls were introduced on the day of FM treatment. Pregnancy to AI was diagnosed 28 to 50 d after AI using ultrasonography. In Exp. 1, beef heifers (n = 1,221) were divided within 5 locations to receive FM or no further treatment (control). At insemination, heifers were divided into 2 similar pastures or pens, and approximately 13 d later, 1 group of heifers within each location was processed through an animal handling facility to administer FM treatment. There was no location x treatment interaction (P = 0.62) on AI pregnancy rates, so data were pooled. Pregnancy rates to AI were reduced (P = 0.02) among heifers receiving the FM treatment procedure (66%) compared with control heifers (72%). In Exp. 2, suckled beef cows (n = 719) were assigned within 2 locations to receive FM or no further treatment (control) approximately 13 d after AI. At insemination, control and FM cows were divided into separate pastures, and only FM cows were handled after AI for the FM treatment procedure. There was no location x treatment interaction (P = 0.75), so data were pooled. Pregnancy rates to AI did not differ (P = 0.80) between FM (57%) and control cows (59%). In Exp 3, beef heifers (n = 247) and suckled beef cows (n = 335) from 1 location received no injection (control) or injection of FM approximately 13 d after AI when all cows and heifers were processed through a working facility. Pregnancy rates to AI were not different (P = 0.37) between FM (45%) and control (42%) cows or between FM (56%) and control (55%) heifers. We conclude FM administration at 1.1 mg/kg of BW approximately 13 d after AI did not improve pregnancy establishment in beef cows and heifers and that the effects of handling heifers at this time may decrease pregnancy establishment.
本研究的目的是确定在人工授精后约 13 天（范围 10 至 15 天）注射 PG 合成抑制剂氟尼辛甲胺（FM；1.1mg/kgBW，肌肉注射）对妊娠建立的影响。使用发情同步的小母牛和奶牛进行了三项实验。在地点和实验内，技术员和人工授精种公牛的处理方式相同。在 FM 处理当天引入公牛。在人工授精后 28 至 50 天使用超声诊断妊娠至人工授精。在实验 1 中，将 1221 头小母牛分为 5 个地点，接受 FM 或不接受进一步处理（对照）。在授精时，小母牛被分为 2 个相似的牧场或围栏，大约 13 天后，每个地点的一组小母牛在动物处理设施中接受 FM 处理。地点 x 处理之间没有相互作用（P = 0.62），因此数据合并。接受 FM 处理程序的小母牛（66%）的妊娠率（P = 0.02）低于对照组小母牛（72%）。在实验 2 中，将 719 头哺乳期奶牛分为 2 个地点，在人工授精后约 13 天接受 FM 或不接受进一步处理（对照）。在授精时，对照组和 FM 奶牛被分为单独的牧场，只有 FM 奶牛在人工授精后接受 FM 处理程序的处理。地点 x 处理之间没有相互作用（P = 0.75），因此数据合并。妊娠率到 AI 没有差异（P = 0.80），FM（57%）和对照牛（59%）。在实验 3 中，来自 1 个地点的 247 头小母牛和 335 头哺乳期奶牛未接受注射（对照）或在人工授精后约 13 天注射 FM，当时所有奶牛和小母牛都在工作设施中进行处理。妊娠率到 AI 没有差异（P = 0.37），FM（45%）和对照（42%）奶牛之间，以及 FM（56%）和对照（55%）小母牛之间。我们得出结论，在人工授精后约 13 天以 1.1mg/kgBW 的剂量注射 FM 不会提高奶牛和小母牛的妊娠建立率，并且此时处理小母牛的效果可能会降低妊娠建立率。
