Guzenko E V, Lemesh V A, Khotyleva L V
Tsitol Genet. 2006 Sep-Oct;40(5):16-20.
The results of reamplification of flax DNA with random primers are represented. It was shown that eight of 17 decameric primers used in the experiments had an reamplification effect: the intensity of the bands increased; new bands and/or new polymorphic bands appeared in the gel sample. The results confirm the effectiveness and necessity of reamplification in case of uncertain or ambiguous interpretation of RAPD analysis making possible to select the highly-informative primers.
展示了用随机引物对亚麻DNA进行再扩增的结果。结果表明，实验中使用的17种十聚体引物中有8种具有再扩增效应：条带强度增加；凝胶样品中出现了新条带和/或新的多态性条带。这些结果证实了在RAPD分析结果不确定或不明确的情况下进行再扩增的有效性和必要性，使得选择高信息量引物成为可能。
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