巴戟天不同农家品种种质资源的随机扩增多态性DNA分析
[RAPD analysis on germplasm resources of different farm races of Morinda officinalis].
作者信息
Ding Ping, Xu Ji-Yin, Chu Tong-Li
机构信息
College of Chinese Materia Medica, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China.
出版信息
Zhong Yao Cai. 2006 Jan;29(1):1-3.
OBJECTIVE
To explore the genetic diversity of different farm races of Morinda officinalis on molecular level.
METHODS
The molecular biological technique-random amplified polymorphic DNA (RAPD) were used.
RESULTS
Of the 40-mer arbitary primers, 14 were found to amplify polymorphic products. 3-5 polymorphic bands were amplified by each polymorphic primer on the average. Using UPGMA method all the tested accesions can be clustered into two groups: 4 accessions of Daye were classified as one group, 1 accession of Xiaoye be another group.
CONCLUSION
There actually existed much genetic diversity on molecular level among the different farm races of Morinda officinalis.
目的
在分子水平上探讨不同农家类型巴戟天的遗传多样性。
方法
采用分子生物学技术——随机扩增多态性DNA（RAPD）。
结果
在40个碱基的随机引物中，筛选出14个能扩增出多态性产物的引物。每个多态性引物平均扩增出3 - 5条多态性条带。采用UPGMA法将所有供试材料聚类为两组：4份大叶巴戟天归为一组，1份小叶巴戟天归为另一组。
结论
不同农家类型巴戟天在分子水平上确实存在较大的遗传多样性。
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