Yu Zhiguo, Gao Xiaoxia, Zhao Yunli, Bi Kaishun
Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, no. 103 Wenhua Road, Shenyang 110016, People's Republic of China.
Biomed Chromatogr. 2007 Jun;21(6):577-84. doi: 10.1002/bmc.764.
A high-performance liquid chromatographic method was developed for the simultaneous determination and pharmacokinetic studies of safflor yellow A, puerarin, 3'-methoxyl-puerarin, and puerarinapioside in the plasma and tissues of rats that had been administered with the traditional Chinese medicine (TCM) preparation Naodesheng via the caudal vein. Samples taken from rats were subjected to protein precipitation with acetone. Separation of these four compounds was accomplished on a Kromisil C18 stationary phase using a mobile phase of acetonitrile-0.1% phosphoric acid-tetrahydrofuran (8:92:2, v/v/v) at a flow-rate of 1.0 mL/min. The detection wavelength was set at 250 nm. The calibration curves of the four components were linear in the given concentration ranges. The intra- and inter-day precisions in plasma and tissues were less than 15% and the extraction recoveries were higher than 60%. The lower limits of quantitation of four components were low enough to determine the four components. These four components all exhibited kinetics that fitted a two-compartment model in rats. The elimination half-life was 1.19 h for safflor yellow A, 2.69 h for puerarin, 2.94 h for 3'-methoxyl-puerarin, and 0.87 h for puerarinapioside, respectively. Following administration of a single injection of Naodesheng, the concentration (C) of the four components in the tissues showed C(kidney) > C(lung), C(liver) > C(spleen), C(stomach), C(heart), approximately. The method is a reliable tool for performing studies of safflor yellow A and three puerarin isoflavones in different biological material.
建立了一种高效液相色谱法，用于同时测定经尾静脉注射中药制剂脑得生的大鼠血浆和组织中红花黄色素A、葛根素、3'-甲氧基葛根素和葛根素木糖苷，并进行药代动力学研究。从大鼠采集的样品用丙酮进行蛋白沉淀。这四种化合物在Kromisil C18固定相上分离，流动相为乙腈-0.1%磷酸-四氢呋喃（8:92:2，v/v/v），流速为1.0 mL/min。检测波长设定为250 nm。四种成分的校准曲线在给定浓度范围内呈线性。血浆和组织中的日内和日间精密度均小于15%，提取回收率高于60%。四种成分的定量下限足够低，能够测定这四种成分。这四种成分在大鼠体内均表现出符合二室模型的动力学特征。红花黄色素A的消除半衰期为1.19 h，葛根素为2.69 h，3'-甲氧基葛根素为2.94 h，葛根素木糖苷为0.87 h。单次注射脑得生后，四种成分在组织中的浓度大致为C(肾)>C(肺)，C(肝)>C(脾)，C(胃)，C(心)。该方法是研究不同生物材料中红花黄色素A和三种葛根素异黄酮的可靠工具。
