Waigmann E, Barta A
Institut für Biochemie, Universität Wien, Vienna, Austria.
Nucleic Acids Res. 1992 Jan 11;20(1):75-81. doi: 10.1093/nar/20.1.75.
Splice sites of vertebrate introns are generally not recognized in plant cells. Several lines of evidences have led to the proposal that the mechanism of 3' splice site selection differs in plants and animals (K. Wiebauer, J.J. Herrero, and W. Filipowicz, Mol. Cell. Biol. 8:2042-2051, 1988). To gain a better insight into the mechanistic differences between plant and animal splicing, we constructed chimeric introns consisting partly of dicotyledonous plant and partly of animal intron sequences. Splicing of these chimeric introns was analyzed in transiently transfected tobacco protoplasts. The results show that there are no principal sequence or structural differences between the 3' splice regions of plants and animals. Furthermore, evidence is provided that cooperation between 5' and 3' splice sites takes place and influences their mutual selection.
脊椎动物内含子的剪接位点在植物细胞中通常无法被识别。多项证据表明，植物和动物中3'剪接位点的选择机制有所不同（K. 维鲍尔、J.J. 埃雷罗和W. 菲利波维茨，《分子与细胞生物学》8:2042 - 2051，1988年）。为了更深入了解植物和动物剪接机制的差异，我们构建了部分由双子叶植物内含子序列和部分由动物内含子序列组成的嵌合内含子。在瞬时转染的烟草原生质体中分析了这些嵌合内含子的剪接情况。结果表明，植物和动物的3'剪接区域在序列或结构上没有主要差异。此外，有证据表明5'和3'剪接位点之间存在协同作用，并影响它们的相互选择。
