使用抑制酪氨酸酶有机相酶传感器分析有机磷和氨基甲酸酯类农药；与丁酰胆碱酯酶+胆碱氧化酶酶电极的比较及在天然水体中的应用

Organophosphorus and carbamate pesticide analysis using an inhibition tyrosinase organic phase enzyme sensor; comparison by butyrylcholinesterase+choline oxidase opee and application to natural waters.

作者信息

Campanella L, Lelo D, Martini E, Tomassetti M

机构信息

Department of Chemistry, University of Rome La Sapienza, P.le Aldo Moro 5, 00185 Rome, Italy.

出版信息

Anal Chim Acta. 2007 Mar 21;587(1):22-32. doi: 10.1016/j.aca.2007.01.023. Epub 2007 Jan 19.

DOI:10.1016/j.aca.2007.01.023

PMID:17386749

Abstract

Recent research performed in our laboratory (using a butyrylcholinesterase+choline oxidase enzyme electrode) suggested the validity of the biosensor approach using enzyme inhibition OPEEs (i.e. enzyme electrodes working in organic phase) in the case of organophosphorus and carbamate pesticides, which are poorly soluble in aqueous solutions. Since these pesticides are generally much more soluble in chloroform than in water, the present research aimed at analysing this class of pesticides using a tyrosinase inhibition OPEE operating in water-saturated chloroform medium. The tyrosinase biosensor was assembled using an oxygen amperometric transducer coupled to the tyrosinase enzyme, immobilized in kappa-carrageenan gel. Lastly a detailed comparison between the inhibition monoenzymatic tyrosinase and inhibition bienzymatic (butyrylcholinesterase+choline oxidase) OPEEs was performed and discussed in this work.

摘要

我们实验室最近开展的研究（使用丁酰胆碱酯酶 + 胆碱氧化酶酶电极）表明，对于在水溶液中溶解度较差的有机磷和氨基甲酸酯类农药，采用酶抑制有机相酶电极（即工作于有机相的酶电极）的生物传感器方法是有效的。由于这些农药通常在氯仿中的溶解度远高于在水中的溶解度，本研究旨在使用在水饱和氯仿介质中工作的酪氨酸酶抑制有机相酶电极来分析这类农药。酪氨酸酶生物传感器是通过将氧电流型换能器与固定在κ-卡拉胶凝胶中的酪氨酸酶相连组装而成。最后，本研究对抑制性单酶酪氨酸酶和抑制性双酶（丁酰胆碱酯酶 + 胆碱氧化酶）有机相酶电极进行了详细比较并加以讨论。