一种用于测定牛奶中三嗪类农药的新型表面等离子体共振免疫传感器：与传统安培型和丝网印刷免疫装置的比较。
A new surface plasmon resonance immunosensor for triazine pesticide determination in bovine milk: a comparison with conventional amperometric and screen-printed immunodevices.
作者信息
Tomassetti Mauro, Martini Elisabetta, Campanella Luigi, Favero Gabriele, Sanzó Gabriella, Mazzei Franco
机构信息
Department of Chemistry, Sapienza University of Rome, P. Le Aldo Moro 5, 00185 Rome, Italy.
Department of Chemistry and Drug Technologies, Sapienza University of Rome, P. Le Aldo Moro 5, 00185 Rome, Italy.
出版信息
Sensors (Basel). 2015 Apr 30;15(5):10255-70. doi: 10.3390/s150510255.
A detailed comparison was made of the analytical features of a new Surface Plasmon Resonance (SPR) immunodevice for triazine pesticide determination with those of two other amperometric (conventional and screen-printed) immunosensors and the advantages and disadvantages of the SPR method were thoroughly investigated. For conventional amperometric and screen-printed devices, "competitive" assays were used; conversely, the SPR transduction technique allowed a "direct" measurement format to be used. As far as the main analytical data are concerned, the SPR method does not seem to offer substantial advantages. Nevertheless the measurement time is much shorter and the measurement itself much easier to perform. Lastly several applications and recovery tests were carried out on bovine milk samples, before and after spiking, to check for triazine pesticides in the samples, obtaining satisfactory results.
对一种用于测定三嗪类农药的新型表面等离子体共振（SPR）免疫装置的分析特性与另外两种安培型（传统型和丝网印刷型）免疫传感器的分析特性进行了详细比较，并深入研究了SPR方法的优缺点。对于传统安培型和丝网印刷型装置，采用了“竞争性”测定法；相反，SPR传感技术允许使用“直接”测量形式。就主要分析数据而言，SPR方法似乎没有显著优势。然而，测量时间要短得多，而且测量本身也更容易进行。最后，对加标前后的牛奶样品进行了几次应用和回收率测试，以检测样品中的三嗪类农药，获得了满意的结果。
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