Wang Xiuyun, Hagiwara Tokio, Uchiyama Shunichi
Department of Materials & Science, Graduate School of Engineering, Saitama Institute of Technology, 1690 Fusaiji Fukaya, Saitama 369-0293, Japan.
Anal Chim Acta. 2007 Mar 21;587(1):41-6. doi: 10.1016/j.aca.2007.01.025. Epub 2007 Jan 19.
A novel amperometric uric acid (UA) sensor has been developed by coating the surface of a gold electrode with a polystyrene (PS) membrane formed by 30 microL of a 30 mg mL(-1) PS chloroform solution combined with 30 microL of a 5 mg mL(-1) polymaleimidostyrene (PMS) solution as a dispersant for enzyme, uricase; this membrane has been successfully employed as an immobilization support for uricase. In the PS membrane, PMS forms micelle-like structures containing uricase in an active state. This immobilized uricase membrane permits the permeation of oxygen, which is consumed by the uricase reaction. A good linear relationship is obtained over the concentration range of 5-105 microM. The concentration of uric acid was determined at a negative potential based on the decrease in the reduction current of oxygen and the interference of l-ascorbic acid can be completely eliminated.
通过用由30微升30毫克/毫升的聚苯乙烯（PS）氯仿溶液与30微升5毫克/毫升的聚马来酰亚胺苯乙烯（PMS）溶液混合形成的聚苯乙烯（PS）膜涂覆金电极表面，开发了一种新型安培型尿酸（UA）传感器，其中PMS溶液用作酶（尿酸酶）的分散剂；该膜已成功用作尿酸酶的固定化载体。在PS膜中，PMS形成含有处于活性状态的尿酸酶的胶束状结构。这种固定化尿酸酶膜允许氧气渗透，氧气在尿酸酶反应中被消耗。在5-105微摩尔的浓度范围内获得了良好的线性关系。基于氧气还原电流的降低在负电位下测定尿酸浓度，并且可以完全消除L-抗坏血酸的干扰。
