McVay Catherine S, Velásquez Marisela, Fralick Joe A
Department of Microbiology and Immunology, Texas Tech University Health Sciences Center, 3601 4th Street, Lubbock, TX 79430, USA.
Antimicrob Agents Chemother. 2007 Jun;51(6):1934-8. doi: 10.1128/AAC.01028-06. Epub 2007 Mar 26.
Mice compromised by a burn wound injury and subjected to a fatal infection with Pseudomonas aeruginosa were administered a single dose of a Pseudomonas aeruginosa phage cocktail consisting of three different P. aeruginosa phages by three different routes: the intramuscular (i.m.), subcutaneous (s.c.), or intraperitoneal (i.p.) route. The results of these studies indicated that a single dose of the P. aeruginosa phage cocktail could significantly decrease the mortality of thermally injured, P. aeruginosa-infected mice (from 6% survival without treatment to 22 to 87% survival with treatment) and that the route of administration was particularly important to the efficacy of the treatment, with the i.p. route providing the most significant (87%) protection. The pharmacokinetics of phage delivery to the blood, spleen, and liver suggested that the phages administered by the i.p. route were delivered at a higher dose, were delivered earlier, and were delivered for a more sustained period of time than the phages administered by the i.m. or s.c. route, which may explain the differences in the efficacies of these three different routes of administration.
对因烧伤伤口损伤而体质受损并遭受铜绿假单胞菌致命感染的小鼠，通过三种不同途径给予单剂量由三种不同铜绿假单胞菌噬菌体组成的铜绿假单胞菌噬菌体鸡尾酒：肌肉注射（i.m.）、皮下注射（s.c.）或腹腔注射（i.p.）途径。这些研究结果表明，单剂量的铜绿假单胞菌噬菌体鸡尾酒可显著降低热损伤、铜绿假单胞菌感染小鼠的死亡率（从未经治疗时的6%存活率提高到治疗后的22%至87%存活率），且给药途径对治疗效果尤为重要，腹腔注射途径提供的保护最为显著（87%）。噬菌体递送至血液、脾脏和肝脏的药代动力学表明，与通过肌肉注射或皮下注射途径给药的噬菌体相比，通过腹腔注射途径给药的噬菌体剂量更高、递送更早且递送持续时间更长，这可能解释了这三种不同给药途径疗效的差异。
