利用高效液相色谱-固相萃取-核磁共振联用技术对新木姜子变种金叶新木姜子中的异喹啉生物碱进行表征分析。

Characterization of isoquinoline alkaloids from Neolitsea sericea var. aurata by HPLC-SPE-NMR.

作者信息

Lee Shoei-Sheng, Lai Yi-Chun, Chen Chien-Kuang, Tseng Li-Hong, Wang Chen-Yu

机构信息

School of Pharmacy, College of Medicine, National Taiwan University, Taipei 100, Taiwan, Republic of China, and Bruker Biospin GmbH, Silberstreifen, D-76287 Rheinstetten, Germany.

出版信息

J Nat Prod. 2007 Apr;70(4):637-42. doi: 10.1021/np060636p. Epub 2007 Mar 28.

DOI:10.1021/np060636p

PMID:17388628

Abstract

Application of the HPLC-SPE-NMR technique to identify the alkaloids in an EtOH extract of the leaves of Neolitsea sericea var. aurata led to the characterization of 14 alkaloids while consuming plant material equivalent to 1.1 g. Of these, seven are N-oxides, four of which are new, namely, 9S,17S-pallidine Nalpha-oxide (6), 1S,2S-reticuline Nalpha-oxide (8), 6R,6aS-boldine Nbeta-oxide (9), and 6S,6aS-N-methyllaurotetanine Nalpha-oxide (13). Their structures were also confirmed by partial synthesis.

摘要

采用高效液相色谱-固相萃取-核磁共振技术对新木姜子变种金叶新木姜子叶乙醇提取物中的生物碱进行鉴定，在消耗相当于1.1 g植物材料的情况下，鉴定出了14种生物碱。其中，7种为N-氧化物，4种为新化合物，即9S,17S-去甲乌药碱Nα-氧化物（6）、1S,2S-网状番荔枝碱Nα-氧化物（8）、6R,6aS-波尔定碱Nβ-氧化物（9）和6S,6aS-N-甲基劳罗他宁Nα-氧化物（13）。它们的结构也通过部分合成得到了证实。