Pelletier G, Li S, Luu-The V, Labrie F
Oncology and Molecular Endocrinology Research Center, Centre de recherche du Centre Hospitalier de l'Université Laval (CRCHUL), Québec, Québec, Canada.
J Neuroendocrinol. 2007 Jun;19(6):426-31. doi: 10.1111/j.1365-2826.2007.01548.x.
It is well documented that oestrogen suppresses food intake by an action at the hypothalamic level. Using in situ hybridisation, we studied the effect of castration (CX) and short-term administration of oestradiol (E2) in CX female mice for three neuropeptides involved in feeding behaviour: two anorexigenic peptides, (i) the pro-opiomelanocortin (POMC)-derived peptide alpha-melanocyte-stimulating hormone and (ii) corticotrophin-releasing hormone (CRH), and the orexigenic peptide, (iii) neuropeptide Y (NPY). POMC-expressing neurones were mostly laterally located in the arcuate nucleus. POMC mRNA expression was decreased following CX and a single injection of E2 induced an increase in mRNA levels at 12- and 24-h time intervals. In the parvocellular area of the paraventricular nucleus, CRH mRNA levels were similarly decreased after CX and completely restored to normal levels at 12 and 24 h following E2 injection. On the other hand, the levels of NPY mRNA expressed in neurones located in the inner zone of the arcuate nucleus were increased by CX and decreased to the levels observed in intact animals by E2 injection (3-24 h). The present data suggest that oestrogen might exert an anorexigenic action by stimulating POMC and CRH mRNA expression and decreasing NPY mRNA expression in the hypothalamus.
有充分文献记载，雌激素通过作用于下丘脑水平来抑制食物摄入。我们采用原位杂交技术，研究了去势（CX）以及对CX雌性小鼠短期给予雌二醇（E2）对三种参与摄食行为的神经肽的影响：两种厌食性肽，（i）源自阿黑皮素原（POMC）的肽α-黑素细胞刺激素和（ii）促肾上腺皮质激素释放激素（CRH），以及促食性肽，（iii）神经肽Y（NPY）。表达POMC的神经元大多位于弓状核的外侧。CX后POMC mRNA表达降低，单次注射E2在12小时和24小时时间间隔时诱导mRNA水平升高。在室旁核的小细胞区，CX后CRH mRNA水平同样降低，E2注射后12小时和24小时完全恢复到正常水平。另一方面，位于弓状核内侧区的神经元中表达的NPY mRNA水平在CX后升高，而通过E2注射（3 - 24小时）降低至完整动物中观察到的水平。目前的数据表明，雌激素可能通过刺激下丘脑POMC和CRH mRNA表达并降低NPY mRNA表达来发挥厌食作用。
