Tashima Cristiano Massao, Tronchini Eleandro Aparecido, Pereira Renata Virginia Fernandes, Bazotte Roberto Barbosa, Zanoni Jacqueline Nelisis
Department of Morpho-Physiological Sciences (DCM/UEM), State University of Maringá, Av. Colombo 5790, 87020-90, Maringá, PR, Brazil.
Dig Dis Sci. 2007 May;52(5):1233-41. doi: 10.1007/s10620-006-9564-8. Epub 2007 Mar 28.
We studied the neuronal density and size of myenteric neurons and the epithelial cell proliferation and crypt depth of the proximal colon in diabetic Wistar rats after supplementing them with L-glutamine (1%). The animals were divided into five groups: untreated normoglycemic (UN), L-glutamine-treated normoglycemic (NG), untreated diabetic (UD), and L-glutamine-treated diabetics 4 days (DG4) and 45 days (DG45) days after the onset of diabetes. We observed a reduction of 52.7% and 50.44% in the neuronal density of the proximal colon of the UD group compared to the UN and NG groups, respectively (P<0.05). The neuronal density found for the DG4 (32.8%) and DG45 (28.6%) groups was higher than that of the UD group (P>0.05). There were no significant differences (P>0.05) when the data relative to the area of the myenteric neuron cell bodies, metaphasic index, and crypt depth in the proximal colon were compared among experimental groups.
我们研究了补充1%L-谷氨酰胺后糖尿病Wistar大鼠的肌间神经元密度和大小，以及近端结肠的上皮细胞增殖和隐窝深度。将动物分为五组：未治疗的正常血糖组（UN）、L-谷氨酰胺治疗的正常血糖组（NG）、未治疗的糖尿病组（UD），以及糖尿病发病后4天（DG4）和45天（DG45）的L-谷氨酰胺治疗糖尿病组。与UN组和NG组相比，我们观察到UD组近端结肠的神经元密度分别降低了52.7%和50.44%（P<0.05）。DG4组（32.8%）和DG45组（28.6%）的神经元密度高于UD组（P>0.05）。当比较实验组近端结肠肌间神经元细胞体面积、中期指数和隐窝深度的数据时，没有显著差异（P>0.05）。
