Tronchini Eleandro A, Trevizan Aline R, Tashima Cristiano M, De Freitas Priscila, Bazotte Roberto B, Pereira Marli A S, Zanoni Jacqueline N
Departamento de Ciências Morfológicas, Universidade Estadual de Maringá, 87020-900 Maringá, PR, Brasil.
An Acad Bras Cienc. 2013 Sep;85(3):1165-76. doi: 10.1590/S0001-37652013005000052. Epub 2013 Aug 28.
The objective of this work was to investigate the effect of the L-glutamine supplementation to prevent - diabetes induced changes in myenteric neurons and also to verify the effect on the mucosa of the ileum of Wistar rats. The animals were divided in five groups (n = 5): untreated normoglycaemic (UN), normoglycaemic treated with L-glutamine (NG), untreated diabetics (UD), diabetics treated with L-glutamine, starting on the 4th (DG4) or 45th day following diabetes induction (DG45). The amino acid was added to the diet at 1%. The density and size of neurons, the metaphasic index in the crypt, the height of the villus, the depth of the crypt and the number of globet cells were determined. There was no difference in the neuronal density and in the cellular body area of the myosin-stained myenteric neurons of groups DG4 and DG45 when compared to group D. The metaphase index and the number of goblet cells showed no significant differences when all groups were compared (P > 0.05). The villi height of groups DG4 and DG45 were 45.5% (P < 0.05) and 32.4% (P > 0.05) higher than those in group UD, respectively. The analyzed crypts showed similar depth for all studied groups.
这项工作的目的是研究补充L-谷氨酰胺对预防糖尿病引起的肌间神经元变化的影响，并验证其对Wistar大鼠回肠黏膜的作用。将动物分为五组（n = 5）：未治疗的正常血糖组（UN）、用L-谷氨酰胺治疗的正常血糖组（NG）、未治疗的糖尿病组（UD）、在诱导糖尿病后第4天（DG4）或第45天（DG45）开始用L-谷氨酰胺治疗的糖尿病组。将氨基酸以1%的比例添加到饮食中。测定神经元的密度和大小、隐窝中的中期指数、绒毛高度、隐窝深度和杯状细胞数量。与糖尿病组（D）相比，DG4组和DG45组中肌球蛋白染色的肌间神经元的神经元密度和细胞体面积没有差异。当比较所有组时，中期指数和杯状细胞数量没有显著差异（P > 0.05）。DG4组和DG45组的绒毛高度分别比UD组高45.5%（P < 0.05）和32.4%（P > 0.05）。所有研究组的分析隐窝显示出相似的深度。
