Li G, Barthelemy A, Feng G, Gentil-Perret A, Peoc'h M, Genin C, Tostain J
Department of Urology, North Hospital, CHU of Saint-Etienne, France.
Histopathology. 2007 Apr;50(5):642-7. doi: 10.1111/j.1365-2559.2007.02655.x.
The common subtypes of renal tumours are conventional, papillary, chromophobe carcinoma and oncocytoma. The morphological differentiation between chromophobe carcinoma and oncocytoma may be difficult. The aim was to evaluate S100A1 as a new marker for the differentiation of the two subtypes.
Thirty-nine tumour samples [nine clear cell renal cell carcinomas (RCCs), six papillary RCCs, nine chromophobe RCCs and 15 oncocytomas] were studied. The protein expression of S100A1 was evaluated by immunohistochemistry. The gene expression of S100A1 was analysed by reverse transcriptase-polymerase chain reaction. Nine oncocytomas showed strong immunoreactivity for S100A1. Four oncocytomas were scored as moderate and one as weak reactivity. In total, 14/15 (93%) of oncocytomas were considered to be immunopositive. In contrast, all nine chromophobe RCCs were considered to be immunonegative. There was a significant difference in the positive percentages of staining of S100A1 between these two subtypes (P < 0.01). S100A1 immunoreactivity was observed in 6/9 clear cell and 4/6 papillary carcinomas. The results of S100A1 gene expression corresponded well with the results of immunohistochemistry.
S100A1 may be a potentially powerful marker to differentiate the chromophobe RCC from renal oncocytoma.
肾肿瘤的常见亚型包括传统型、乳头状、嫌色细胞癌和嗜酸细胞瘤。嫌色细胞癌和嗜酸细胞瘤之间的形态学鉴别可能存在困难。本研究旨在评估S100A1作为这两种亚型鉴别的新标志物。
研究了39个肿瘤样本[9例透明细胞肾细胞癌（RCC）、6例乳头状RCC、9例嫌色细胞RCC和15例嗜酸细胞瘤]。采用免疫组织化学法评估S100A1的蛋白表达。通过逆转录-聚合酶链反应分析S100A1的基因表达。9例嗜酸细胞瘤对S100A1呈强免疫反应性。4例嗜酸细胞瘤评分为中度反应性，1例为弱反应性。总计14/15（93%）的嗜酸细胞瘤被认为免疫阳性。相比之下，所有9例嫌色细胞RCC均被认为免疫阴性。这两种亚型之间S100A1染色阳性百分比存在显著差异（P < 0.01）。在6/9的透明细胞癌和4/6的乳头状癌中观察到S100A1免疫反应性。S100A1基因表达结果与免疫组织化学结果良好对应。
S100A1可能是区分肾嫌色细胞癌和肾嗜酸细胞瘤的一种潜在有力标志物。
