Sarpietro Maria Grazia, Spatafora Carmela, Tringali Corrado, Micieli Dorotea, Castelli Francesco
Dipartimento di Scienze Chimiche, Università degli Studi di Catania, Viale Andrea Doria 6, 95125 Catania, Italy.
J Agric Food Chem. 2007 May 2;55(9):3720-8. doi: 10.1021/jf070070q. Epub 2007 Mar 31.
The interaction of resveratrol (trans-3,5,4'-trihydroxystilbene) and two of its derivatives (3,5,4'-tri-O-methylresveratrol and 3,5,4'-tri-O-triacetylresveratrol) with biomembrane models, represented by dimyristoylphosphatidylcholine (DMPC) multilamellar vesicles (MLV), has been studied by differential scanning calorimetry (DSC). The analysis of MLV prepared in the presence of increasing molar fraction of such compounds has been carried out to reveal their maximum interaction with biomembrane models. The results from these studies have been compared with kinetic experiments results, in order to detect the entity and rate of compound absorption by the biomembrane models. The findings indicate that the compounds affected the thermotropic properties of DMPC MLV by suppressing the pretransition peak and broadening the DMPC main phase transition calorimetric peak and shifting it to lower temperatures. The order of effectiveness found was resveratrol > trimethylresveratrol > triacetylresveratrol. The kinetic experiments reveal that in an aqueous medium the absorption of resveratrol by the biomembranes models is allowed, whereas the absorption of its derivatives is hindered; in contrast when a lipophilic medium is employed, all three compounds are easily absorbed.
通过差示扫描量热法（DSC）研究了白藜芦醇（反式-3,5,4'-三羟基芪）及其两种衍生物（3,5,4'-三-O-甲基白藜芦醇和3,5,4'-三-O-三乙酰白藜芦醇）与以二肉豆蔻酰磷脂酰胆碱（DMPC）多层囊泡（MLV）为代表的生物膜模型的相互作用。对在这些化合物摩尔分数不断增加的情况下制备的MLV进行分析，以揭示它们与生物膜模型的最大相互作用。将这些研究结果与动力学实验结果进行比较，以检测生物膜模型对化合物的吸收程度和速率。研究结果表明，这些化合物通过抑制预转变峰、拓宽DMPC主相变热谱峰并将其移至较低温度，影响了DMPC MLV的热致性质。发现的有效性顺序为白藜芦醇>三甲基白藜芦醇>三乙酰白藜芦醇。动力学实验表明，在水性介质中，生物膜模型允许吸收白藜芦醇，而其衍生物的吸收受到阻碍；相反，当使用亲脂性介质时，所有三种化合物都易于吸收。
