Timm C D, Irino K, Gomes T A T, Vieira M M, Guth B E C, Vaz T M I, Moreira C N, Aleixo J A G
Programa de Pós-graduação em Ciência e Tecnologia Agroindustrial, UFPel, Pelotas, RS, Brazil.
Lett Appl Microbiol. 2007 Apr;44(4):419-25. doi: 10.1111/j.1472-765X.2006.02085.x.
To determine the prevalence of Shiga toxin-producing Escherichia coli (STEC) and serotypes and virulence markers of the STEC isolates from beef and dairy cattle in Rio Grande do Sul, Brazil.
Faecal samples from beef cattle were collected at slaughterhouses. The isolates were submitted to colony hybridization assay with specific DNA probes for stx1, stx2 and eae genes, and serotyped for the identification of O and H antigens. Thirty-nine per cent of beef cattle surveyed harboured at least one STEC strain. Among the distinct serotypes identified, 10 were shared by both beef and dairy cattle. Most of the strains isolated harboured stx2. Genotypic and phenotypic profiles allowed the identification of 34 and 31 STEC strains, isolated from beef and dairy cattle, respectively. Serotypes O10:H14, O15:H21, O96:H21, O119:H4, O124:H11, O128:H21, O137:H-, O141:H19, O159:H42, O160:H2 and O177:H11, identified in this study, have not been previously reported as STEC isolated from cattle.
Cattle are an important reservoir of STEC strains associated with human diseases in South America.
Determining the prevalence, genotypic profile and serotypes of STEC strains isolated from cattle enables the prediction of possible risk for public health.
确定巴西南里奥格兰德州肉牛和奶牛中产志贺毒素大肠杆菌（STEC）的流行率、血清型以及STEC分离株的毒力标记。
在屠宰场采集肉牛的粪便样本。将分离株用stx1、stx2和eae基因的特异性DNA探针进行菌落杂交试验，并对O和H抗原进行血清分型鉴定。受调查的肉牛中有39%携带至少一种STEC菌株。在鉴定出的不同血清型中，有10种是肉牛和奶牛共有的。分离出的大多数菌株携带stx2。通过基因型和表型分析分别从肉牛和奶牛中鉴定出34株和31株STEC菌株。本研究中鉴定出的血清型O10:H14、O15:H21、O96:H21、O119:H4、O124:H11、O128:H21、O137:H-、O141:H19、O159:H42、O160:H2和O177:H11，此前尚未报道为从牛中分离出的STEC。
牛是南美洲与人类疾病相关的STEC菌株的重要储存宿主。
确定从牛中分离出的STEC菌株的流行率、基因型和血清型有助于预测对公共卫生可能存在的风险。
