Chen Pei-Jen, Kullman Seth W, Hinton David E, Linden Karl G
National Health and Environmental Effects Research Laboratory, EPA, RTP, NC, USA.
Chemosphere. 2007 Jun;68(6):1041-9. doi: 10.1016/j.chemosphere.2007.02.020. Epub 2007 Mar 29.
Polychromatic ultraviolet irradiation, such as from medium pressure (MP) Hg lamps may enhance the UV degradation of environmental pollutants as compared to low pressure (LP) Hg UV sources emitting monochromatic irradiation. Typically, studies involving destruction of environmental pollutants such as endocrine disrupting compounds (EDCs) are based on measurement of the parent compound decay using analytical chemistry, but such information is insufficient to determine an effective treatment endpoint because the identity and biological activity of many transformation products remain unknown. Bioanalytical methods to assess residual biological activity of a treated water offers one means to compare removal efficiency of EDC activity between MP- and LP-UV lamps under photolysis and UV/H2O2 oxidation. In this study, changes in estrogenic activity of bisphenol-A (BPA) as a function of UV treatment were evaluated using both an in vitro yeast estrogen screen and in vivo vitellogenin assay with Japanese medaka (Oryzias latipes) fish. Decay of BPA parent compound and formation of degradation products were followed using HPLC analysis. Results demonstrated that MP-UV direct photolysis more effectively removed BPA and associated estrogenic activity compared to LP-UV lamps. UV in combination with H2O2 significantly removed estrogenic activity in vitro and in vivo compared to direct photolysis; however, no significant difference in removal rates was found between the two lamps under UV/H2O2 oxidation. Furthermore, the UV/H2O2 process was effective for reducing embryo toxicity of BPA, but resulted in the production of acidic intermediates, causing acute toxicity and delayed hatching in some medaka embryos.
与发射单色辐射的低压（LP）汞紫外光源相比，多色紫外辐射，如来自中压（MP）汞灯的辐射，可能会增强环境污染物的紫外降解。通常，涉及破坏环境污染物（如内分泌干扰化合物（EDC））的研究是基于使用分析化学测量母体化合物的衰减，但这些信息不足以确定有效的处理终点，因为许多转化产物的身份和生物活性仍然未知。评估处理后水中残留生物活性的生物分析方法提供了一种手段，用于比较MP-UV灯和LP-UV灯在光解和UV/H2O2氧化条件下对EDC活性的去除效率。在本研究中，使用体外酵母雌激素筛选和体内卵黄蛋白原测定法，以日本青鳉（Oryzias latipes）鱼评估双酚A（BPA）雌激素活性随紫外线处理的变化。使用高效液相色谱（HPLC）分析跟踪BPA母体化合物的衰减和降解产物的形成。结果表明，与LP-UV灯相比，MP-UV直接光解能更有效地去除BPA及其相关的雌激素活性。与直接光解相比，UV与H2O2联合使用在体外和体内均能显著去除雌激素活性；然而，在UV/H2O2氧化条件下，两种灯之间的去除率没有显著差异。此外，UV/H2O2工艺对降低BPA的胚胎毒性有效，但会产生酸性中间体，导致一些青鳉胚胎出现急性毒性和孵化延迟。
