Shaw Andrew E, Reid Scott M, Ebert Katja, Hutchings Geoffrey H, Ferris Nigel P, King Donald P
Institute for Animal Health, Pirbright Laboratory, Ash Road, Pirbright, Woking, Surrey, GU24 0NF, United Kingdom.
J Virol Methods. 2007 Jul;143(1):81-5. doi: 10.1016/j.jviromet.2007.02.009. Epub 2007 Mar 29.
An automated one-step real-time reverse transcription polymerase chain reaction (rRT-PCR) protocol was optimised and evaluated for the routine diagnosis of foot-and-mouth disease (FMD). Parallel testing of RNA samples (n=257) indicated that this assay has a diagnostic sensitivity at least equivalent to the automated two-step rRT-PCR protocol previously used for the laboratory detection of FMD virus (FMDV). This more rapid and economical one-step protocol will play a key role in contingency planning for any future outbreaks of FMD in the United Kingdom (UK).
一种用于口蹄疫（FMD）常规诊断的自动化一步法实时逆转录聚合酶链反应（rRT-PCR）方案得到了优化和评估。对RNA样本（n = 257）进行的平行检测表明，该检测方法的诊断敏感性至少等同于先前用于实验室检测口蹄疫病毒（FMDV）的自动化两步法rRT-PCR方案。这种更快速且经济的一步法方案将在英国（UK）未来任何口蹄疫疫情的应急规划中发挥关键作用。
