National Centre for Foreign Animal Disease, Canadian Food Inspection Agency, Winnipeg, MB R3E 3M4, Canada.
The Pirbright Institute, Woking GU24 0NF, UK.
Viruses. 2022 Apr 10;14(4):785. doi: 10.3390/v14040785.
Foot-and-mouth disease virus (FMDV) causes FMD, a highly contagious disease of cloven-hoofed animals including cattle, goats, pigs and sheep. Rapid detection of FMDV is critical to limit the devastating economic losses due to FMD. Current laboratory methods for FMDV detection such as virus isolation, real-time reverse transcription PCR and antigen detection enzyme-linked immunosorbent assay (AgELISA) are labor-intensive, requiring trained personnel and specialized equipment. We present the development and validation of a pan-serotype lateral flow strip test (LFST) that uses recombinant bovine integrin αvβ6 as a universal capture ligand and a pan-serotype monoclonal antibody (mAb) to detect FMDV. The LFST detected all seven FMDV serotypes, where the diagnostic sensitivity was comparable to the AgELISA, and the diagnostic specificity was 100% without cross-reactivity to other viruses causing vesicular disease in livestock. This rapid test will be useful for on-site FMDV detection, as well as in laboratories in endemic countries where laboratory resources are limited.
口蹄疫病毒（FMDV）引起口蹄疫，这是一种高度接触传染性疾病，会感染包括牛、山羊、猪和绵羊在内的偶蹄动物。快速检测 FMDV 对于限制口蹄疫造成的破坏性经济损失至关重要。目前用于 FMDV 检测的实验室方法，如病毒分离、实时逆转录聚合酶链反应和抗原检测酶联免疫吸附试验（AgELISA），劳动强度大，需要经过培训的人员和专用设备。我们开发并验证了一种 pan-serotype 侧向流动条检测（LFST），该检测使用重组牛整合素 αvβ6 作为通用捕获配体和 pan-serotype 单克隆抗体（mAb）来检测 FMDV。LFST 检测到了所有七种 FMDV 血清型，其诊断灵敏度与 AgELISA 相当，诊断特异性为 100%，与引起家畜水疱病的其他病毒无交叉反应。这种快速检测方法将有助于现场 FMDV 检测，以及在实验室资源有限的流行国家的实验室中使用。
