Tada M, Omata M, Ohto M
First Department of Medicine, Chiba University School of Medicine, Japan.
Cancer. 1992 Mar 1;69(5):1115-8. doi: 10.1002/cncr.2820690509.
The DNA sequences around codons 12, 13, and 61 of the ras gene were analyzed by polymerase chain reaction and direct sequencing in 18 intrahepatic cholangiocarcinomas. The ras gene mutations were found in 9 of 18 (50%): 6 in K-ras codon 12, 1 in K-ras codon 13, 1 in K-ras codon 61, and 1 in N-ras codon 12. The incidence of mutations was higher in the hilar type of intrahepatic cholangiocarcinomas, especially when these tumors were large. The incidence and spectrum of the mutations were almost the same as those reported in colon cancers, possibly indicating similar etiologic agent(s) in the carcinogenesis of both cancers.
通过聚合酶链反应和直接测序法，对18例肝内胆管癌中ras基因第12、13和61密码子周围的DNA序列进行了分析。在18例中有9例（50%）发现了ras基因突变：K-ras第12密码子突变6例，K-ras第13密码子突变1例，K-ras第61密码子突变1例，N-ras第12密码子突变1例。肝门部肝内胆管癌的突变发生率更高，尤其是肿瘤体积较大时。这些突变的发生率和谱与结肠癌中报道的几乎相同，这可能表明两种癌症在致癌过程中有相似的病因。
