Tada M, Omata M, Ohto M
First Department of Medicine, Chiba University School of Medicine.
Nihon Shokakibyo Gakkai Zasshi. 1990 Mar;87(3):811-5.
Ras genes are converted to active oncogenes by point mutations occurring in either codon 12, 13 or 61. We used polymerase chain reaction and direct sequence method for the analysis of these mutations. We examined 13 hepatocellular carcinomas, 8 cholangiocarcinomas, 2 hepatoblastomas and 1 biliary cystadenocarcinoma. Of these tumors, ras gene mutations were detected solely in cholangiocarcinomas. Cholangiocarcinoma showed a high incidence of K-ras gene mutation. Among 8 patients with cholangiocarcinoma, the mutation was detected at codon 12 in 4 and at codon 61 in 1. The incidence of K-ras gene mutation was especially high in the hilar type of cholangiocarcinoma as compared with the peripheral type.
Ras基因通过发生在密码子12、13或61的点突变转化为活性癌基因。我们采用聚合酶链反应和直接测序法分析这些突变。我们检测了13例肝细胞癌、8例胆管癌、2例肝母细胞瘤和1例胆管囊腺癌。在这些肿瘤中，仅在胆管癌中检测到ras基因突变。胆管癌显示出较高的K-ras基因突变发生率。在8例胆管癌患者中，4例在密码子12处检测到突变，1例在密码子61处检测到突变。与周围型胆管癌相比，肝门型胆管癌的K-ras基因突变发生率尤其高。
