Cauglia F, Canepa P
Environmental Technology Center, P.zza De Ferrari, 16100 Genoa, Italy.
Bioresour Technol. 2008 Jul;99(10):4065-72. doi: 10.1016/j.biortech.2007.01.036. Epub 2007 Mar 30.
'Sugar esters' are non-ionic biodegradable surfactant which are potentially attractive for the cosmetic and food market. Unfortunately, the formation of by-products by chemical synthesis affects the quality of the surfactant. Enzymatic synthesis is a promising and environmentally friendly approach preventing this problem but requiring a proper setting up of the reaction system (membrane reactor, azeotropic mixture, pervaporation system, etc.) for the control of the water activity so that the enzyme efficiency is often negatively affected. In this paper, the synthesis of glucosylmyristate by Novozym 435 is taken as a model to illustrate the general features of the 'sugar esters' synthesis, with particularly regard to the influence on the synthesis of the pre-reaction treatment of the enzyme and reaction mixture, water adsorbents, reaction solvents, products and reagents. The aim is reached by placing the water adsorbents in contact with the enzyme preparation, so that high dehydration efficiency is achieved and the other factors affecting the kinetic of the synthesis are better highlighted.
“糖酯”是非离子型可生物降解表面活性剂，对化妆品和食品市场具有潜在吸引力。不幸的是，化学合成过程中副产物的形成会影响表面活性剂的质量。酶促合成是一种有前景且环保的方法，可避免这一问题，但需要合理设置反应系统（膜反应器、共沸混合物、渗透蒸发系统等）来控制水分活度，因此酶的效率常常受到负面影响。本文以诺维信435催化合成肉豆蔻酸葡萄糖酯为例，阐述“糖酯”合成的一般特征，尤其关注酶和反应混合物的预反应处理、吸水剂、反应溶剂、产物及试剂对合成的影响。通过使吸水剂与酶制剂接触来实现这一目标，从而获得较高的脱水效率，并更好地突出影响合成动力学的其他因素。
