九里香胰蛋白酶抑制剂的结晶及初步X射线衍射研究。
Crystallization and preliminary X-ray diffraction studies of Murraya koenigii trypsin inhibitor.
作者信息
Shee Chandan, Singh Tej P, Kumar Pravindra, Sharma Ashwani K
机构信息
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Apr 1;63(Pt 4):318-9. doi: 10.1107/S1744309107011414. Epub 2007 Mar 23.
Abstract
A Kunitz-type trypsin inhibitor purified from the seeds of Murraya koenigii has been crystallized by the sitting-drop vapour-diffusion method using PEG 8000 as the precipitating agent. The crystals belong to the tetragonal space group P4(3)2(1)2, with unit-cell parameters a = b = 75.8, c = 150.9 A. The crystals contain two molecules in the asymmetric unit with a V(M) value of 2.5 A(3) Da(-1). Diffraction was observed to 2.65 A resolution and a complete data set was collected to 2.9 A resolution.
摘要
从九里香种子中纯化得到的一种Kunitz型胰蛋白酶抑制剂，采用悬滴气相扩散法，以聚乙二醇8000作为沉淀剂进行了结晶。晶体属于四方晶系空间群P4(3)2(1)2，晶胞参数a = b = 75.8，c = 150.9 Å。不对称单元中含有两个分子，V(M)值为2.5 ų Da⁻¹。观察到衍射分辨率为2.65 Å，并收集了分辨率为2.9 Å的完整数据集。
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