Liphaus B L, Kiss M H B, Goldberg A C
Unidade de Reumatologia, Instituto da Criança, Universidade de São Paulo, Brasil.
Braz J Med Biol Res. 2007 Apr;40(4):591-7. doi: 10.1590/s0100-879x2007000400019.
Human leukocyte antigens (HLA) DRB103 and DRB102 have been associated with systemic lupus erythematosus (SLE) in Caucasians and black populations. It has been observed that certain HLA alleles show stronger associations with SLE autoantibodies and clinical subsets, although they have rarely been associated with lupus renal histologic class. In the present study, HLA-DRB1 allele correlations with clinical features, autoantibodies and renal histologic class were analyzed in a cohort of racially mixed Brazilian patients with juvenile-onset SLE. HLA-DRB1 typing was carried out by polymerase chain reaction amplification with sequence-specific primers using genomic DNA from 55 children and adolescents fulfilling at least four of the American College of Rheumatology criteria for SLE. Significance was determined by the chi-square test applied to 2 x 2 tables. The HLA-DRB115 allele was most frequent in patients with renal, musculoskeletal, cutaneous, hematologic, cardiac, and neuropsychiatric involvement, as well as in patients positive for anti-dsDNA, anti-Sm, anti-U1-RNP, and anti-SSA/Ro antibodies, although an association between HLA alleles and SLE clinical features and autoantibodies could not be observed. The HLA-DRB117, HLA-DRB110, HLA-DRB115, and HLA-DRB1*07 alleles were significantly higher in patients with renal histologic class I, class IIA, class IIB, and class V, respectively. The present results suggest that the contribution of HLA- DRB1 alleles to juvenile-onset SLE could not be related to clinical or serological subsets of the disease, but it may be related to renal histologic classes, especially class I, class II A, class II B, and class V. The latter correlations have not been observed in literature.
人类白细胞抗原（HLA）DRB103和DRB102与白种人和黑人人群中的系统性红斑狼疮（SLE）相关。据观察，某些HLA等位基因与SLE自身抗体和临床亚组的关联性更强，尽管它们很少与狼疮肾组织学类型相关。在本研究中，分析了一组种族混合的巴西青少年SLE患者中HLA - DRB1等位基因与临床特征、自身抗体和肾组织学类型的相关性。采用序列特异性引物的聚合酶链反应扩增技术，对55名符合美国风湿病学会SLE标准至少四项的儿童和青少年的基因组DNA进行HLA - DRB1分型。通过应用于2×2表格的卡方检验确定显著性。HLA - DRB115等位基因在有肾脏、肌肉骨骼、皮肤、血液、心脏和神经精神受累的患者中最为常见，在抗双链DNA、抗Sm、抗U1 - RNP和抗SSA/Ro抗体阳性的患者中也最为常见，尽管未观察到HLA等位基因与SLE临床特征和自身抗体之间的关联。HLA - DRB117、HLA - DRB110、HLA - DRB115和HLA - DRB1*07等位基因分别在肾组织学类型为I类、IIA类、IIB类和V类的患者中显著更高。目前的结果表明，HLA - DRB1等位基因对青少年SLE的影响可能与该疾病的临床或血清学亚组无关，但可能与肾组织学类型有关，特别是I类、IIA类、IIB类和V类。后者的相关性在文献中尚未见报道。
