日本系统性红斑狼疮患者的HLA - DRB1等位基因与β2糖蛋白I依赖性抗心磷脂抗体

HLA-DRB1 alleles and beta 2 glycoprotein I-dependent anticardiolipin antibodies in Japanese patients with systemic lupus erythematosus.

作者信息

Hashimoto H, Yamanaka K, Tokano Y, Iida N, Takasaki Y, Kabasawa K, Nishimura Y

机构信息

Department of Internal Medicine and Rheumatology, Juntendo University, Tokyo, Japan.

出版信息

Clin Exp Rheumatol. 1998 Jul-Aug;16(4):423-7.

PMID:9706422

Abstract

OBJECTIVE

To investigate the association of HLA DRB1 alleles with beta 2 glycoprotein I (beta 2 GPI)-dependent anticardiolipin antibodies (aCL) in Japanese patients with systemic lupus erythematosus (SLE).

METHODS

One hundred and forty-five Japanese patients with SLE were studied. beta 2 GPI-dependent aCL was measured by enzyme-linked immunosorbent assays. DNA typing of the DRB1 alleles was performed by the polymerase chain reaction sequence specific oligonucleotide probe method.

RESULTS

beta 2 GPI-dependent aCL was positive in 29 (20.0%) out of 145 SLE patients. SLE patients with beta 2 GPI-dependent aCL had a significantly higher frequency or one or more of the clinical manifestations assumed to be associated with aCL, compared to those without beta 2 GPI-dependent aCL (p < 0.05). The frequency of DRB10901 was lower in SLE patients than in healthy subjects. SLE patients with beta 2 GPI-dependent aCL were significantly associated with DRB10901 as compared to those without beta 2 GPI-dependent aCL (41.4% vs 15.5%, p < 0.005, R.R. = 3.8), although the corrected P value was not significant.

CONCLUSION

A possible association of DRB1*0901 with Japanese SLE patients with beta 2 GPI-dependent aCL was found. This association indicates an association between the disease and the HLA-DR53 (DRB4)-bearing haplotypes in different ethnic groups.

摘要

目的

研究日本系统性红斑狼疮（SLE）患者中人类白细胞抗原DRB1等位基因与β2糖蛋白I（β2GPI）依赖性抗心磷脂抗体（aCL）之间的关联。

方法

对145例日本SLE患者进行研究。采用酶联免疫吸附测定法检测β2GPI依赖性aCL。通过聚合酶链反应序列特异性寡核苷酸探针法对DRB1等位基因进行DNA分型。

结果

145例SLE患者中，29例（20.0%）β2GPI依赖性aCL呈阳性。与无β2GPI依赖性aCL的SLE患者相比，有β2GPI依赖性aCL的SLE患者出现一种或多种假定与aCL相关的临床表现的频率显著更高（p<0.05）。SLE患者中DRB10901的频率低于健康受试者。与无β2GPI依赖性aCL的SLE患者相比，有β2GPI依赖性aCL的SLE患者与DRB10901显著相关（41.4%对15.5%，p<0.005，相对危险度=3.8），尽管校正后的P值不显著。