2,5-二羟基苯甲酸和维生素A对高氧诱导大鼠脑抗氧化作用的评估
Assessment of antioxidant effect of 2,5-dihydroxybenzoic acid and vitamin a in brains of rats with induced hyperoxia.
作者信息
Calderón Guzmán David, Trujillo Jiménez Francisca, Hernández García Ernestina, Juárez Olguín Hugo
机构信息
Laboratorio de Neuroquímica, Instituto Nacional de Pediatría, Mexico City, Mexico.
出版信息
Neurochem Res. 2007 Jun;32(6):1036-40. doi: 10.1007/s11064-006-9269-6. Epub 2007 Mar 31.
The aim of this study was to evaluate the effect of 2,5-dihydroxybenzoic acid, a salicylate derived from Acetyl salicylic acid (ASA) and vitamin A (vit A) on Na(+), K(+) ATPase enzyme and GSH levels in brain of rats exposed to hyperoxia (Hyp) as oxidant protocol. Rats were treated as follow: group I (control), group II (Hyp), group III (Hyp, ASA), group IV (vit A), group V (Hyp, vit A), group VI (Hyp, vit A, ASA). Vit A was given 5 days before and during Hyp, aspirin at the end of Hyp. Na(+),K(+) ATPase and total ATPase activity was significantly increased in group V. Levels of GSH showed a significant increase in group III, besides, levels of 2,5-dihydroxybenzoic acid as salicylate in plasma were significantly increased in group II. These results elucidate differences in the biochemical response of animal towards intake of various types of antioxidant substances, with increased GSH and salicylate in hyperoxia.
本研究的目的是评估2,5 - 二羟基苯甲酸（一种由乙酰水杨酸（ASA）和维生素A（维生素A）衍生的水杨酸盐）对暴露于高氧（Hyp）作为氧化方案的大鼠脑中Na(+)、K(+) - ATP酶和谷胱甘肽（GSH）水平的影响。大鼠的处理方式如下：第一组（对照组），第二组（高氧组），第三组（高氧组、ASA），第四组（维生素A组），第五组（高氧组、维生素A），第六组（高氧组、维生素A、ASA）。在高氧暴露前5天及高氧暴露期间给予维生素A，在高氧暴露结束时给予阿司匹林。第五组中Na(+)、K(+) - ATP酶和总ATP酶活性显著增加。第三组中GSH水平显著升高，此外，第二组中血浆中水杨酸盐形式的2,5 - 二羟基苯甲酸水平显著增加。这些结果阐明了动物对摄入各种类型抗氧化物质的生化反应差异，在高氧状态下GSH和水杨酸盐增加。
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