Garrisi G J, Talansky B E, Sapira V, Gordon J W, Navot D
Department of Obstetrics, Gynecology, and Reproductive Science, Mount Sinai School of Medicine, New York, New York.
Fertil Steril. 1992 Mar;57(3):677-81. doi: 10.1016/s0015-0282(16)54920-6.
To determine the developmental potential of mouse embryos that underwent cryopreservation after micromanipulation of the zona pellucida.
Gaps were produced in the zona pellucida of mouse oocytes or two-cell embryos by zona drilling with acid Tyrode's solution. Zona-drilled oocytes were fertilized in vitro and cultured to the two-cell stage. Two-cell embryos were frozen, thawed, and cultured to the expanded blastocyst stage.
There was no difference in the rate of embryo survival post-thaw (248/318, 77% versus 288/345, 83.4%), or in the rate of development to the expanded blastocyst stage (91/248, 36.7% versus 88/288, 30.6%), between embryos that were zona drilled as oocytes and unmanipulated controls. Similarly, there was no difference in the rate of cryosurvival (206/217, 94.9% versus 168/187, 89.8%) or development to the blastocyst stage (154/206, 74.7% versus 132/168, 78.6%) between embryos that were fertilized in vivo and zona drilled at the two-cell stage and embryos that were unmanipulated.
These findings indicate that small gaps in the zona pellucida, such as those that result from micromanipulation, do not significantly alter the ability of embryos to withstand cryopreservation.
确定在对透明带进行显微操作后经冷冻保存的小鼠胚胎的发育潜能。
用酸性台氏液对小鼠卵母细胞或二细胞胚胎的透明带打孔，制造缺口。对打孔的卵母细胞进行体外受精并培养至二细胞期。将二细胞胚胎冷冻、解冻并培养至扩张囊胚期。
作为卵母细胞时进行透明带打孔的胚胎与未处理的对照胚胎相比，解冻后胚胎存活率（248/318，77% 对 288/345，83.4%）以及发育至扩张囊胚期的比率（91/248，36.7% 对 88/288，30.6%）均无差异。同样，体内受精并在二细胞期进行透明带打孔的胚胎与未处理的胚胎相比，冷冻存活率（206/217，94.9% 对 168/187，89.8%）或发育至囊胚期的比率（154/206，74.7% 对 132/168，78.6%）也无差异。
这些发现表明，透明带上的小缺口，如显微操作造成的缺口，不会显著改变胚胎耐受冷冻保存的能力。
