Jensen A, Hagelskjaer Kristensen L, Prag J
Department of Clinical Microbiology, Viborg Hospital, Viborg, Denmark.
Clin Microbiol Infect. 2007 Jul;13(7):695-701. doi: 10.1111/j.1469-0691.2007.01719.x. Epub 2007 Apr 2.
Throat swabs from 61 patients, aged 18-32 years, with non-streptococcal tonsillitis (NST) and 92 healthy controls were examined for the presence of Fusobacterium necrophorum DNA using a novel TaqMan-based real-time quantitative PCR assay for F. necrophorum subspecies. The assay was based on the gyrB subunit gene, and detected F. necrophorum DNA in 48% of patients with NST and in 21% of controls (p <0.001). F. necrophorum subsp. funduliforme was the only subspecies found in both patients and controls. The load of F. necrophorum DNA on swabs from patients with NST was significantly higher than that on swabs from controls (p <0.001). Furthermore, patients with recurrent NST had a significantly higher load of F. necrophorum DNA compared to patients with acute NST (p 0.04). In addition, 26 patients with tonsillitis and group C streptococci (GCS) had a significantly higher load of F. necrophorum DNA compared to the NST group (p <0.001). It was concluded that F. necrophorum subsp. funduliforme is present in small numbers as part of the normal human throat flora, and that F. necrophorum in large quantities may cause tonsillitis, especially recurrent tonsillitis. In addition, the study suggests that the concomitant presence of GCS may aggravate F. necrophorum tonsillitis.
对61名年龄在18至32岁之间患有非链球菌性扁桃体炎（NST）的患者以及92名健康对照者的咽拭子进行检测，采用一种基于新型TaqMan的实时定量PCR检测法检测坏死梭杆菌亚种的DNA。该检测法基于gyrB亚基基因，在48%的NST患者和21%的对照者中检测到坏死梭杆菌DNA（p<0.001）。坏死梭杆菌亚种funduliforme是在患者和对照者中均发现的唯一亚种。NST患者咽拭子上的坏死梭杆菌DNA载量显著高于对照者咽拭子上的载量（p<0.001）。此外，与急性NST患者相比，复发性NST患者的坏死梭杆菌DNA载量显著更高（p=0.04）。另外，与NST组相比，26名患有扁桃体炎且感染C组链球菌（GCS）的患者的坏死梭杆菌DNA载量显著更高（p<0.001）。研究得出结论，坏死梭杆菌亚种funduliforme作为正常人类咽喉菌群的一部分少量存在，而大量的坏死梭杆菌可能导致扁桃体炎，尤其是复发性扁桃体炎。此外，该研究表明GCS的同时存在可能会加重坏死梭杆菌性扁桃体炎。
