Huang Y H, Tseng S P, Hu J M, Tsai J C, Hsueh P R, Teng L J
Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, Taipei, Taiwan.
Clin Microbiol Infect. 2007 Jul;13(7):717-24. doi: 10.1111/j.1469-0691.2007.01718.x. Epub 2007 Apr 2.
The staphylococcal chromosome cassette (SCC)mec types of 382 hospital-acquired methicillin-resistant Staphylococcus aureus (HA-MRSA) isolates in Taiwan were analysed over a 7-year period (1999-2005). There was an abrupt increase in SCCmec type IV in HA-MRSA during 2005. The molecular epidemiology of a subset (n = 69) of HA-MRSA isolates with SCCmec types III, IV or V was characterised and compared with that of community-acquired MRSA (CA-MRSA) (n = 26, collected during 2005). Pulsed-field gel electrophoresis revealed three major pulsotypes (A, B and C) and 15 minor clones. Pulsotypes B and C, which contained isolates carrying SCCmec types IV and V, respectively, included both CA-MRSA and HA-MRSA isolates. Among 24 toxin genes analysed, five genes had significant differential distribution between CA-MRSA and SCCmec type III HA-MRSA. Furthermore, among SCCmec type IV isolates, the seb gene was detected more commonly in HA-MRSA. Analysis of representative members of the three major pulsotypes by multilocus sequence typing revealed two sequence types (STs), namely ST239 (SCCmecIII) and ST59 (SCCmecIV or SCCmecV). This suggests that ST59:SCCmecIV, which is usually community-acquired, has become an important nosocomial pathogen in the hospital studied.
在7年期间（1999 - 2005年），对台湾382株医院获得性耐甲氧西林金黄色葡萄球菌（HA - MRSA）分离株的葡萄球菌染色体盒式（SCC）mec型进行了分析。2005年期间，HA - MRSA中SCCmec IV型突然增加。对一部分具有SCCmec III型、IV型或V型的HA - MRSA分离株（n = 69）的分子流行病学特征进行了分析，并与社区获得性MRSA（CA - MRSA，2005年收集，n = 26）进行了比较。脉冲场凝胶电泳揭示了三种主要脉冲型（A、B和C）和15个次要克隆。脉冲型B和C分别包含携带SCCmec IV型和V型的分离株，包括CA - MRSA和HA - MRSA分离株。在分析的24个毒素基因中，有5个基因在CA - MRSA和SCCmec III型HA - MRSA之间存在显著差异分布。此外，在SCCmec IV型分离株中，seb基因在HA - MRSA中更常见。通过多位点序列分型对三种主要脉冲型的代表性菌株进行分析，揭示了两种序列类型（STs），即ST239（SCCmecIII）和ST59（SCCmecIV或SCCmecV）。这表明通常为社区获得性的ST59:SCCmecIV已成为所研究医院中的一种重要医院感染病原体。
