Roy Joannie, Faktorová Drahomíra, Benada Oldrich, Lukes Julius, Burger Gertraud
Centre Robert Cedergren, Bioinformatics & Genomics, Département de biochimie, Université de Montréal, Montréal, QC, Canada H3T 1J4.
J Eukaryot Microbiol. 2007 Mar-Apr;54(2):137-45. doi: 10.1111/j.1550-7408.2007.00244.x.
We describe Rhynchopus euleeides n. sp., using light and electron microscopy. This free-living flagellate, which was isolated earlier from a marine habitat, can be grown axenically in a rich medium based on modified seawater. In the trophic stage, cells are predominantly elliptical and laterally flattened, but frequently change their shape (metaboly). Gliding is the predominant manner of locomotion. The two flagella, which are typically concealed in their pocket, are short stubs of unequal length, have conventional axonemes, but apparently lack a paraxonemal rod. Swarmer cells, which form only occasionally, are smaller in size and carry two conspicuous flagella of more than 2 times the body length. Cells are decorated with a prominent apical papillum. Both the flagellar pocket and the adjacent feeding apparatus seem to merge together into a single sub-apical opening. The mitochondrion, which is most likely single, is located peripherally. It is reticulated in shape and contains only a few lamellar cristae. Mitochondrial DNA is abundant and evenly distributed throughout the organelle. Morphological synapomorphies confirm the affiliation of the species with the genus Rhynchopus (Diplonemea, Euglenozoa). We discuss the characters that distinguish Rhynchopus from Diplonema corroborating the validity of the two genera.
我们利用光学显微镜和电子显微镜描述了新物种尤氏喙鞭毛虫（Rhynchopus euleeides n. sp.）。这种自由生活的鞭毛虫先前从海洋生境中分离得到，可在基于改良海水的丰富培养基中无菌培养。在营养阶段，细胞主要呈椭圆形且侧向扁平，但形状经常改变（变形运动）。滑行是主要的运动方式。两条鞭毛通常隐藏在它们的袋囊中，是长度不等的短残端，具有传统的轴丝，但显然缺少副轴丝杆。仅偶尔形成的游动细胞尺寸较小，带有两条明显长于体长两倍的鞭毛。细胞顶端有一个突出的乳头状突起。鞭毛袋和相邻的摄食装置似乎合并成一个单一的亚顶端开口。线粒体很可能是单个的，位于周边。其形状呈网状，仅含有少数板层嵴。线粒体DNA丰富且均匀分布于整个细胞器。形态学共衍征证实了该物种与喙鞭毛虫属（Diplonemea，眼虫纲）的归属关系。我们讨论了区分喙鞭毛虫属与双鞭毛虫属的特征，确证了这两个属的有效性。
