Roy Joannie, Faktorová Drahomíra, Lukes Julius, Burger Gertraud
Centre Robert Cedergren, Bioinformatics & Genomics, Département de biochimie, Université de Montréal, Montréal, QC, Canada, H3 T 1J4.
Protist. 2007 Jul;158(3):385-96. doi: 10.1016/j.protis.2007.03.002. Epub 2007 May 11.
Mitochondrial DNA of Kinetoplastea is composed of different chromosomes, the maxicircle (bearing 'regular' genes) and numerous minicircles (specifying guide RNAs involved in RNA editing). In trypanosomes [Kinetoplastea], DNA circles are compacted into a single dense body, the kinetoplast. This report addresses the question whether multi-chromosome mitochondrial genomes and compacted chromosome organization are restricted to Kinetoplastea or rather occur throughout Euglenozoa, i.e., Kinetoplastea, Euglenida and Diplonemea. To this end, we investigated the diplonemid Rhynchopus euleeides and the euglenids Petalomonas cantuscygni, Peranema trichophorum and Entosiphon sulcatum, using light and electron microscopy and molecular techniques. Our findings together with previously published data show that multi-chromosome mitochondrial genomes prevail across Euglenozoa, while kinetoplast-like mtDNA packaging is confined to trypanosomes.
动基体目生物的线粒体DNA由不同的染色体组成，即大圆环（携带“常规”基因）和众多小圆环（指定参与RNA编辑的引导RNA）。在锥虫（动基体目）中，DNA环被压缩成一个单一的致密体，即动基体。本报告探讨了多染色体线粒体基因组和压缩染色体组织是仅限于动基体目，还是在整个眼虫门中都存在，即动基体目、裸藻目和双鞭毛虫目。为此，我们利用光学显微镜、电子显微镜和分子技术研究了双鞭毛虫Rhynchopus euleeides以及裸藻Petalomonas cantuscygni、Peranema trichophorum和Entosiphon sulcatum。我们的研究结果与之前发表的数据共同表明，多染色体线粒体基因组在眼虫门中普遍存在，而动基体样的线粒体DNA包装仅限于锥虫。
