Liu D X, Wada A, Huang L
Department of Biochemistry, University of Tennessee, Knoxville.
Immunol Lett. 1992 Feb;31(2):177-81. doi: 10.1016/0165-2478(92)90143-c.
We have recently described that large liposomes composed of egg phosphatidylcholine (PC), cholesterol (chol) and monosialoganglioside GM1 show elevated accumulation in the red pulp of the spleen when they are i.v. administered into mice. Up to 50% of the injected dose was found in spleen at 4 h post injection. In this report, we have investigated the potential application of such liposomes in the stimulation of anti-lysozyme response in mice. Lysozyme entrapped in the splenotropic liposomes composed of PC/chol/GM1 showed higher efficiency in potentiating the humoral response than that of either free lysozyme or lysozyme entrapped in hepatotropic liposomes composed of PC/chol. The results demonstrate that high levels of i.v. antigen delivery by liposomes to the splenic macrophage instead of the liver Kupffer cells is important in the liposomal adjuvanticity. The antibody elicited by the liposome entrapped antigen was mainly IgG1 subtype.
我们最近报道，由鸡蛋磷脂酰胆碱（PC）、胆固醇（chol）和单唾液酸神经节苷脂GM1组成的大脂质体经静脉注射到小鼠体内后，在脾脏红髓中的蓄积量会增加。注射后4小时，脾脏中发现的注射剂量高达50%。在本报告中，我们研究了此类脂质体在刺激小鼠抗溶菌酶反应方面的潜在应用。包裹在由PC/chol/GM1组成的脾靶向脂质体中的溶菌酶，在增强体液反应方面比游离溶菌酶或包裹在由PC/chol组成的肝靶向脂质体中的溶菌酶具有更高的效率。结果表明，脂质体将静脉内抗原高效递送至脾巨噬细胞而非肝库普弗细胞，这在脂质体佐剂效应中很重要。脂质体包裹抗原引发的抗体主要是IgG1亚型。
