van Rooijen N, van Nieuwmegen R
Acta Histochem. 1979;65(1):41-8. doi: 10.1016/S0065-1281(79)80031-8.
Attempts were made to study the localization of intravenously injected liposomes and enclosed labelled antigens in the spleen of mice, using autoradiography. A more than hundred fold increased uptake of antigen by the spleen was obtained when liposome entrapped antigen was compared with free antigen or antigen-antibody complexes which have been used in earlier studies. No marked accumulation of the labelled antigen was found in the follicles of the spleen, although specific antibodies to the injected antigen have been injected simultaneously with the liposome entrapped antigen. It is argued that the bulk of the labelled antigen in the spleen 0.5 to 2 h after injection was still enclosed in the liposomes, whereas part of the label, arranged in patches in the red pulp and marginal zone, corresponds with labelled antigen in macrophages, released after digestion of the liposomal membranes. The present techniques did not enable the detection of the liposomes themselves.
利用放射自显影技术，尝试研究静脉注射脂质体及包封于其中的标记抗原在小鼠脾脏中的定位。与早期研究中使用的游离抗原或抗原-抗体复合物相比，脂质体包裹的抗原使脾脏对抗原的摄取增加了一百多倍。尽管在注射脂质体包裹抗原的同时也注射了针对该注射抗原的特异性抗体，但在脾脏滤泡中未发现标记抗原的明显积聚。有人认为，注射后0.5至2小时，脾脏中大部分标记抗原仍包裹在脂质体中，而在红髓和边缘区呈斑块状分布的部分标记物，与脂质体膜消化后释放到巨噬细胞中的标记抗原相对应。目前的技术无法检测到脂质体本身。
