Bertelsmann Holger, Sieme Harald, Behne Dietrich, Kyriakopoulos Antonios
Hahn-Meitner Institut, Department of Molecular Trace Element Research in the Life Science, SF 6, Glienickerstr. 100, 14109 Berlin, Germany.
Ann N Y Acad Sci. 2007 Jan;1095:204-8. doi: 10.1196/annals.1397.024.
In the sperm nuclei, of mammalian species selenium has been found only in the form of sperm nuclei glutathione peroxidase (snGPx) where it is most likely bound to the chromatin of spermatozoa. Over 80% of selenium in sperm is bound to the selenoprotein phospholipid hydroperoxide glutathione peroxidase (PHGPx) in the midpiece of rat sperm. Zinc in sperm is mainly contained in the outer dense fiber (ODF) proteins of the flagella of mammalian spermatozoa. In the sperm nuclei, zinc is predominately located in the chromatin to the protamine proteins. In order to investigate if the insertion of zinc and selenium in sperm chromatin is regulated, the element concentrations were determined in equine spermatozoa and purified sperm nuclei. We found a significant positive correlation between the selenium concentration in equine spermatozoa and sperm nuclei. The same finding was obtained for the zinc concentration in spermatozoa and sperm nuclei. The results assume that the distribution of selenium and zinc in spermatozoa is regulated by cell signaling pathways and in this way determining the selenium and zinc amount in the chromatin of spermatozoa.
在哺乳动物精子细胞核中，仅在精子细胞核谷胱甘肽过氧化物酶（snGPx）形式中发现硒，它很可能与精子染色质结合。大鼠精子中段超过80%的硒与硒蛋白磷脂氢过氧化物谷胱甘肽过氧化物酶（PHGPx）结合。精子中的锌主要存在于哺乳动物精子鞭毛的外致密纤维（ODF）蛋白中。在精子细胞核中，锌主要位于与鱼精蛋白结合的染色质中。为了研究锌和硒在精子染色质中的插入是否受到调节，我们测定了马精子和纯化精子细胞核中的元素浓度。我们发现马精子和精子细胞核中的硒浓度之间存在显著正相关。精子和精子细胞核中的锌浓度也得到了相同的结果。结果表明，精子中硒和锌的分布受细胞信号通路调节，从而决定精子染色质中的硒和锌含量。
