Samyn Nele, Laloup Marleen, De Boeck Gert
Laboratory of Toxicology, National Institute of Criminalistics and Criminology (N.I.C.C.), Federal Public Service Justice, Vilvoordsesteenweg 100, 1120 Brussels, Belgium.
Anal Bioanal Chem. 2007 Aug;388(7):1437-53. doi: 10.1007/s00216-007-1245-8. Epub 2007 Apr 3.
Recent advances in analytical techniques have enabled the detection of drugs and drug metabolites in oral fluid specimens. Although GC-MS is still commonly used in practice, many laboratories have developed and successfully validated methods for LC-MS(-MS) that can detect a large number of compounds in the limited sample volume available. In addition, several enzyme immunoassays have been commercialized for the detection of drugs of abuse in oral fluid samples, enabling the fast screening and selection of presumably positive samples. A number of concerns are discussed, such as the variability in the volume of sample collected and its implications in terms of quantitative measurements, and the drug recoveries of the many different specimen collection systems on the market. Additional considerations that also receive attention are the importance of providing complete validation data with respect to analyte stability, matrix effect, and the choice of collection method.
分析技术的最新进展使得能够在口腔液样本中检测药物和药物代谢物。尽管气相色谱 - 质谱联用（GC-MS）在实际应用中仍被广泛使用，但许多实验室已经开发并成功验证了液相色谱 - 质谱（ - 质谱联用）（LC-MS(-MS)）方法，该方法能够在有限的样本体积中检测大量化合物。此外，几种酶免疫测定法已商业化用于检测口腔液样本中的滥用药物，从而能够对可能呈阳性的样本进行快速筛查和筛选。文中讨论了一些问题，例如所采集样本体积的变异性及其对定量测量的影响，以及市场上许多不同样本采集系统的药物回收率。还受到关注的其他考虑因素包括提供关于分析物稳定性、基质效应和采集方法选择的完整验证数据的重要性。
