Ben Amar A, Cobanov P, Boonrod K, Krczal G, Bouzid S, Ghorbel A, Reustle G M
Laboratoire de Physiologie Moléculaire de la Vigne, Technopôle de Borj Cédria, BP 95, Hammam-Lif 2050, Tunisia.
Plant Cell Rep. 2007 Sep;26(9):1439-47. doi: 10.1007/s00299-007-0341-8. Epub 2007 Apr 3.
An efficient system for the establishment and multiplication of highly prolific embryogenic cell cultures of grapevine (Vitis sp.) was developed. Using anther-derived pro-embryogenic masses as starting material, cell suspensions of different grapevine cultivars (Tempranillo, Cabernet-Sauvignon) and rootstocks (Kober 125 AA, Kober 5 BB, 110 Richter) were initiated in liquid medium containing NOA (1.0 mg l(-1)) and BAP (0.25 mg l(-1)) as growth regulators. Conditioned medium was recovered and utilised for establishing new, highly totipotent cell cultures. The suspensions obtained, showed embryogenic competence resulting in somatic embryo induction and subsequent plant regeneration. In this study, a simplified establishment procedure for grapevine embryogenic cell suspension allowing the fast multiplication of embryogenic material is described. Evidence for the promoting effect of the protein fraction derived from conditioned medium, on cell proliferation was found. In bioassays, addition of ss-D: -GlcY affect cell proliferation suggesting that arabinogalactan proteins are required for growth processes in grapevine cell cultures.
开发了一种高效的系统，用于建立和增殖葡萄（Vitis sp.）高度高产的胚性细胞培养物。以花药来源的原胚性团块为起始材料，在含有萘氧乙酸（NOA，1.0 mg l(-1)）和苄氨基嘌呤（BAP，0.25 mg l(-1)）作为生长调节剂的液体培养基中，启动了不同葡萄品种（丹魄、赤霞珠）和砧木（考贝125AA、考贝5BB、110里希特）的细胞悬浮液。回收条件培养基并用于建立新的、高度全能的细胞培养物。获得的悬浮液表现出胚性能力，导致体细胞胚诱导和随后的植株再生。在本研究中，描述了一种简化的葡萄胚性细胞悬浮液建立程序，可实现胚性材料的快速增殖。发现了条件培养基中蛋白质组分对细胞增殖的促进作用的证据。在生物测定中，添加β-D-葡萄糖醛酸影响细胞增殖，表明阿拉伯半乳聚糖蛋白是葡萄细胞培养生长过程所必需的。
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