从胚性组织中分离的葡萄原生质体的植株再生。

Plant regeneration of grapevine (Vitis sp.) protoplasts isolated from embryogenic tissue.

机构信息

Department of Viticulture (370), University of Hohenheim, D-70593, Stuttgart, Germany.

出版信息

Plant Cell Rep. 1995 Dec;15(3-4):238-41. doi: 10.1007/BF00193727.

DOI:10.1007/BF00193727

Abstract

Protoplasts with high embryogenic competence could be isolated from leaf-disk-derived embryos and embryoids of Vitis sp. cv. Seyval blanc. After a 4-week induction treatment in NN-69 medium supplemented with 4.0mg/l naphthoxyacetic acid (NOA) and 0.9mg/l thidiazuron (TDZ) and subsequent subcultivation in hormone-free medium, 38.5% of the developed microcalluses showed somatic embryogenesis. In contrast, only few formed somatic embryos after induction in CPW-13 medium with either 1.0mg/l 2,4-dichlorophenoxyacetic acid and 0.5mg/l benzylaminopurine treatment (13.8%) or NOA/TDZ treatment (1.4%). Up to 30% of these embryos germinated and about half of them regenerated into typical in vitro grapevines when transferred onto LS-medium in culture tubes.

摘要

原生质体具有较高的胚胎发生能力，可以从葡萄属品种 Seyval blanc 的叶片圆盘衍生的胚胎和胚状体中分离出来。在 NN-69 培养基中添加 4.0mg/L 萘氧乙酸（NOA）和 0.9mg/L 噻二唑隆（TDZ）进行 4 周的诱导处理，随后在无激素培养基中进行继代培养后，38.5%的发育小愈伤组织表现出体细胞胚胎发生。相比之下，在 CPW-13 培养基中分别用 1.0mg/L 2,4-二氯苯氧乙酸和 0.5mg/L 苄氨基嘌呤（13.8%）或 NOA/TDZ（1.4%）处理后，只有少数小愈伤组织诱导形成体细胞胚。这些胚中，有高达 30%的胚发芽，其中约一半在培养管中的 LS 培养基中转移后再生为典型的离体葡萄植株。