Jelski Wojciech, Chrostek Lech, Laszewicz Wiktor, Szmitkowski Maciej
Department of Biochemical Diagnostics, Medical University, Waszyngtona 15 A, Bialystok, Poland.
Dig Dis Sci. 2007 Jun;52(6):1513-6. doi: 10.1007/s10620-006-9566-6. Epub 2007 Apr 3.
Human gastric mucosa contains three classes of alcohol dehydrogenase (ADH) isoenzymes: I, III, and IV. Various factors have been found to influence gastric ADH activity. One of them is Helicobacter pylori infection, which is associated with gastric mucosal injury and leads to a decrease in gastric ADH activity. The aim of the study was to assess the effect of H. pylori infection on the serum activity of ADH isoenzymes. Serum samples were taken from 35 patients with H. pylori infection and from 35 healthy subjects. For measurement of class I isoenzyme activity we employed the fluorometric method, with class-specific fluorogenic substrate (4-methoxy-1-naphthaldehyde). The activities of class III and IV ADH isoenzymes were measured by the photometric method with formaldehyde and with m-nitrobenzaldehyde as substrate, respectively. Total activity of ADH was measured by a photometric method with p-nitrosodimethylaniline. The total activities of ADH and class IV isoenzyme were significantly higher in sera of patients with H. pylori infection compared to healthy subjects. The serum activity of other tested isoenzymes of ADH did not differ significantly between infected and noninfected groups. We conclude that H. pylori infection of gastric mucosa is reflected in the serum by a significant increase in class IV and total ADH activity.
人类胃黏膜含有三类乙醇脱氢酶（ADH）同工酶：I、III和IV。已发现多种因素会影响胃ADH活性。其中之一是幽门螺杆菌感染，它与胃黏膜损伤相关，并导致胃ADH活性降低。本研究的目的是评估幽门螺杆菌感染对ADH同工酶血清活性的影响。从35例幽门螺杆菌感染患者和35名健康受试者中采集血清样本。对于I类同工酶活性的测定，我们采用荧光法，使用类特异性荧光底物（4-甲氧基-1-萘甲醛）。III类和IV类ADH同工酶的活性分别通过以甲醛和间硝基苯甲醛为底物的比色法测定。ADH的总活性通过以对亚硝基二甲基苯胺的比色法测定。与健康受试者相比，幽门螺杆菌感染患者血清中ADH和IV类同工酶的总活性显著更高。感染组和未感染组之间其他检测的ADH同工酶的血清活性没有显著差异。我们得出结论，胃黏膜的幽门螺杆菌感染在血清中表现为IV类和总ADH活性显著增加。
