Kohl W, Witte B, Höfle G
Gesellschaft für Biotechnologische Forschung mbH, Abt. Niedermolekulare Naturstoffe, Braunschweig.
Planta Med. 1983 Mar;47(3):177-82. doi: 10.1055/s-2007-969980.
HPLC separation conditions for the qualitative and quantitative analysis of indole alkaloids from Catharanthus roseus tissue cultures were discribed as well as sample preparation procedures with Extrelut and Bond Elut SCX cartridges, which allow a quicker and more effective sample clean up as the classical alkaline chloroform extraction method. The methods are suitable for very small amounts of cell material.
描述了用于长春花组织培养中吲哚生物碱定性和定量分析的高效液相色谱（HPLC）分离条件，以及使用Extrelut和Bond Elut SCX柱的样品制备程序，与传统的碱性氯仿萃取法相比，这些程序能实现更快、更有效的样品净化。这些方法适用于极少量的细胞材料。
