Department of Agronomy and Soil Science, University of New England, 2351, Armidale, N.S.W., Australia.
J Chem Ecol. 1993 Oct;19(10):2245-54. doi: 10.1007/BF00979661.
A method was devised for the extraction and quantification of hordenine and gramine from barley (Hordeum vulgare) tissue using HPLC techniques. Quantification was by peak area, the relationship between peak area and concentration of authentic standards being linear for both hordenine and gramine. Significant differences in the ability of three lines of barley to produce hordenine and gramine were detected using this method.
采用 HPLC 技术,设计了一种从大麦(Hordeum vulgare)组织中提取和定量霍多宁和禾谷胺的方法。通过峰面积进行定量,霍多宁和禾谷胺的峰面积与标准品浓度之间的关系均呈线性。使用该方法检测到三种大麦品系产生霍多宁和禾谷胺能力的显著差异。