Kataoka Takahiro, Mizuguchi Yuko, Notohara Kenji, Taguchi Takehito, Yamaoka Kiyonori
Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho, 2-chome, Okayama 700-8558, Japan.
Physiol Chem Phys Med NMR. 2006;38(1):21-9.
We have previously determined by using immune-assay or bio-assay methods that low-dose irradiation enhances immune and anti-oxidation functions. In this study, we examined histological changes of lymphatic follicles at 4, 24, or 48 hrs after sham, 0.25, 0.5, or 15 Gy irradiation in the spleens of BALB/c mice, which are sensitive to radiation compared with other strains, and C57BL/6J mice, which are resistant to radiation, using hematoxylin-eosin staining for lymphatic follicles or methylgreen pyronin staining for plasma cells. Results show that the lymphatic follicles in the spleens of the two mouse strains decreased at 24 or 48 hrs after 15 Gy irradiation. The number of plasma cells in the spleens of sham irradiated BALB/c mice was greater than that of sham irradiated C57BL/6J mice. At 4 hrs after 0.25 Gy irradiation, plasma cells increased in the spleens of the two mouse strains. These findings suggest, by histology, that low-dose irradiation activates the plasma cells and enhances the immune function. Although those two mouse strains have different sensitivities to radiation, the above changes were similar in both time course and degree of response. Therefore, the phenomena observed may be common in mice.
我们之前通过免疫测定或生物测定方法确定，低剂量辐射可增强免疫和抗氧化功能。在本研究中，我们使用苏木精-伊红染色观察淋巴滤泡或甲基绿派洛宁染色观察浆细胞，检查了对辐射敏感的BALB/c小鼠和对辐射有抗性的C57BL/6J小鼠在假照射、0.25、0.5或15 Gy照射后4、24或48小时脾脏中淋巴滤泡的组织学变化。结果显示，在15 Gy照射后24或48小时，两种小鼠品系脾脏中的淋巴滤泡减少。假照射的BALB/c小鼠脾脏中的浆细胞数量多于假照射的C57BL/6J小鼠。在0.25 Gy照射后4小时，两种小鼠品系脾脏中的浆细胞增加。这些组织学结果表明，低剂量辐射激活浆细胞并增强免疫功能。尽管这两种小鼠品系对辐射的敏感性不同，但上述变化在时间进程和反应程度上相似。因此，观察到的现象可能在小鼠中普遍存在。
