Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho, 2-chome, Okayama 700-8558, Japan.
J Clin Biochem Nutr. 2009 Sep;45(2):219-26. doi: 10.3164/jcbn.09-06. Epub 2009 Aug 28.
We previously reported that low-dose X-irradiation alleviates ischemia-reperfusion injury such as mouse paw edema. In this study, we examined active changes in the biological function of mouse liver grafts in cold storage after low-dose X-irradiation. Mouse livers were sham-irradiated or were irradiated with 0.25, 0.5, 1.0, or 5.0 Gy of X-ray and stored for 4, 8, 24, or 48 h in preservation or saline solution. The results show that storage for 24 h in saline solution after 0.5 Gy irradiation significantly increased the activity of superoxide dismutase (SOD) and catalase. Following storage for 4, 8, or 48 h in preservation solution, lipid peroxide levels of the 0.5 Gy irradiated group were significantly lower than those of the sham irradiated group. Following storage for 24 h in preservation solution, the activity of SOD and catalase of the 1.0 Gy irradiated group were significantly higher than those of the sham irradiated group. Hepatocytes stored in saline solution were vacuolated. However, no vacuole formation was observed in hepatocytes stored in preservation solution. These findings suggest that low-dose irradiation significantly activates antioxidative functions of liver grafts. Moreover, the dose at which enhancement of antioxidative function occurs in livers stored in preservation solution, which contains glutathione, is significantly higher than that in saline solution.
我们之前报道过低剂量 X 射线照射可以减轻如小鼠爪肿胀等缺血再灌注损伤。在这项研究中，我们检测了低剂量 X 射线照射后小鼠肝移植物在冷藏过程中生物功能的主动变化。将小鼠肝脏假照射或用 0.25、0.5、1.0 或 5.0 Gy 的 X 射线照射，并在保存液或盐水中储存 4、8、24 或 48 小时。结果表明，在 0.5 Gy 照射后在盐水中储存 24 小时可显著增加超氧化物歧化酶（SOD）和过氧化氢酶的活性。在保存液中储存 4、8 或 48 小时后，0.5 Gy 照射组的脂质过氧化物水平明显低于假照射组。在保存液中储存 24 小时后，1.0 Gy 照射组的 SOD 和过氧化氢酶活性明显高于假照射组。储存在盐水中的肝细胞出现空泡化。然而，储存在含有谷胱甘肽的保存液中的肝细胞未观察到空泡形成。这些发现表明，低剂量照射可显著激活肝移植物的抗氧化功能。此外，在含有谷胱甘肽的保存液中，肝脏抗氧化功能增强的剂量明显高于盐水。
