Lynch Jeremy A, Desplan Claude
Department of Biology, New York University, 100 Washington Square East, New York, New York 10003, USA.
Nat Protoc. 2006;1(1):486-94. doi: 10.1038/nprot.2006.70.
The wasp Nasonia vitripennis is emerging as a useful model organism in which to address a variety of biological questions, due, in part, to its ease of laboratory use, unique aspects of its biology and the sequencing of its genome. In order to take full advantage of the potential of this organism, methods for manipulating gene function are needed. To this end, a protocol for parental RNA interference (pRNAi) in N. vitripennis is described. pRNAi entails injecting pupae with double-stranded RNA, allowing the injected wasps to eclose and examining the progeny for developmental defects. This basic protocol is described in the context of the life cycle of N. vitripennis. This technique has been useful in elucidating the function of most, although not all, genes tested to date, and has potential applications beyond embryonic patterning. pRNAi experiments in Nasonia can be completed in as little as 2 weeks.
丽蝇蛹集金小蜂正成为一种有用的模式生物，可用于解决各种生物学问题，部分原因在于其易于在实验室使用、独特的生物学特性以及基因组测序。为了充分利用这种生物的潜力，需要操纵基因功能的方法。为此，本文描述了一种丽蝇蛹集金小蜂亲代RNA干扰（pRNAi）的方案。pRNAi包括向蛹注射双链RNA，使注射后的黄蜂羽化，并检查后代是否存在发育缺陷。该基本方案是在丽蝇蛹集金小蜂的生命周期背景下进行描述的。这项技术在阐明迄今为止测试的大多数（尽管不是全部）基因的功能方面很有用，并且在胚胎模式形成之外还有潜在的应用。丽蝇蛹集金小蜂的pRNAi实验最短可在2周内完成。
