Smith Alison M, Zeeman Samuel C
Department of Metabolic Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom.
Nat Protoc. 2006;1(3):1342-5. doi: 10.1038/nprot.2006.232.
This protocol describes a simple means of measuring the starch content of plant tissues by solubilizing the starch, converting it quantitatively to glucose and assaying the glucose. Plant tissue must initially be frozen rapidly to stop metabolism, then extracted to remove free glucose. Starch is solubilized by heating, then digested to glucose by adding glucan hydrolases. Glucose is assayed enzymatically. The method is more sensitive and accurate than iodine-based protocols, and is suitable for tissues that have a wide range of starch contents. Measurements on multiple samples can be completed within a day.
本方案描述了一种通过溶解淀粉、将其定量转化为葡萄糖并测定葡萄糖来测量植物组织淀粉含量的简单方法。植物组织必须首先迅速冷冻以停止代谢，然后进行提取以去除游离葡萄糖。淀粉通过加热溶解，然后加入葡聚糖水解酶消化成葡萄糖。葡萄糖通过酶法测定。该方法比基于碘的方案更灵敏、更准确，适用于淀粉含量范围广泛的组织。多个样品的测量可在一天内完成。
