De Simoni Anna, Yu Lily M Y
Department of Physiology, University College London, Gower Street, London WCIE 6BT, UK.
Nat Protoc. 2006;1(3):1439-45. doi: 10.1038/nprot.2006.228.
This protocol describes a method for making and culturing rat hippocampal organotypic slices on membrane inserts. Supplementary videos are included to demonstrate visually the different steps of the procedure. Cultured hippocampal slices has been increasingly used as a model for synaptic studies of the brain as they allow examination of mid to long term manipulations in a preparation where the gross cytoarchitecture of the hippocampus is preserved. Combining techniques such as molecular biology, electrophysiology and immunohistochemistry to study physiological or pathological processes can easily be applied to organotypic slices. The technique described here can be used to make organotypic slices from other parts of the brain, other rodent species and from a range of ages. This protocol can be completed in 3 h.
本方案描述了一种在膜插入物上制作和培养大鼠海马体器官型切片的方法。包含补充视频以直观展示该过程的不同步骤。培养的海马体切片越来越多地被用作大脑突触研究的模型，因为它们允许在保留海马体大体细胞结构的制剂中检查中长期操作。将分子生物学、电生理学和免疫组织化学等技术结合起来研究生理或病理过程可以很容易地应用于器官型切片。这里描述的技术可用于从大脑的其他部位、其他啮齿动物物种以及不同年龄段制作器官型切片。本方案可在3小时内完成。
