Weiss Yael, Rubin Baruch, Shulman Avidor, Ben Shir Irina, Keinan Ehud, Wolf Shmuel
The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University, Rehovot 76100, Israel.
Nat Protoc. 2006;1(5):2282-7. doi: 10.1038/nprot.2006.380.
Herbicide-resistant plants can be generated by either traditional breeding procedures or genetic engineering. Analyses of plant responses to a newly developed herbicide or the tolerance level of a newly developed plant line to a given herbicide are based on various bioassays. Here, we describe several methods for quantitative measurements of plants' responses to propham application, as a model herbicide of the carbamate family. Dose-response assays include seed germination and analyses of shoot and root elongation on paper. To better reflect the natural interaction between the plant, the soil and the herbicide, a protocol for germination and root elongation on sand is described. Finally, a more sensitive bioassay is based on plant growth on agar medium. The described protocols are simple, reproducible and can be easily adopted for a variety of plant species and for various herbicides. Plants' response to a given herbicide can be determined within a few weeks.
抗除草剂植物可以通过传统育种程序或基因工程来培育。对植物对新开发除草剂的反应或新开发植物品系对特定除草剂的耐受水平的分析基于各种生物测定法。在此，我们描述了几种用于定量测量植物对氨基甲酸酯类模型除草剂苯胺灵施用反应的方法。剂量反应测定包括种子发芽以及在纸上对地上部和根部伸长的分析。为了更好地反映植物、土壤和除草剂之间的自然相互作用，我们描述了一种在沙子上进行发芽和根伸长的方案。最后，一种更灵敏的生物测定法基于植物在琼脂培养基上的生长情况。所描述的方案简单、可重复，并且可以很容易地应用于各种植物物种和各种除草剂。植物对特定除草剂的反应可以在几周内确定。
