Yamanouye Norma, Kerchove Celine Marie, Moura-da-Silva Ana Maria, Carneiro Sylvia M, Markus Regina P
Laboratório de Farmacologia, Instituto Butantan, Avenida Vital Brazil, 1500, 05503-900, São Paulo, Brazil.
Nat Protoc. 2006;1(6):2763-6. doi: 10.1038/nprot.2006.423.
This protocol details the optimal conditions to establish a long-term primary culture of secretory cells from the venom gland of the Bothrops jararaca snake. Furthermore, these conditions allow the production and secretion of venom into the culture medium. Snake venom is a rich source of active molecules and has been used for bioprospection studies. However, obtaining enough venom from snakes is a major obstacle. Secretory cells of venom glands are capable of producing active toxins. Therefore, a culture of secretory cells is a good in vitro system to acquire the venom of snakes without capturing the animal from the wild. The protocol described here provides a rapid (approximately 4 h) and reproducible means of producing sufficient amounts of snake venom for biological investigations.
本方案详细介绍了从巴西矛头蝮蛇毒腺建立分泌细胞长期原代培养的最佳条件。此外，这些条件可使毒液在培养基中产生和分泌。蛇毒是活性分子的丰富来源，已用于生物勘探研究。然而，从蛇身上获取足够的毒液是一个主要障碍。毒腺的分泌细胞能够产生活性毒素。因此，分泌细胞培养是一种无需从野外捕获动物就能获取蛇毒的良好体外系统。这里描述的方案提供了一种快速（约4小时）且可重复的方法，用于生产足够量的蛇毒以进行生物学研究。
