MacLeod Roderick A F, Kaufmann Maren, Drexler Hans G
DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
Nat Protoc. 2007;2(2):372-82. doi: 10.1038/nprot.2007.29.
Tumor cell lines are widely used both as disease models and, increasingly, as genomic resources for the ascertainment of new cancer genes. Cytogenetic analysis remains a major route to uncovering the cancer genome. However, cancer cell lines vary inexplicably in their harvesting preferences, which must, therefore, be determined by trial and error. This article describes harvesting protocols optimized empirically for 550 commonly used, mainly human, cancer cell lines together with evidence-based procedures to assist in determining conditions for unlisted cell lines and subsidiary protocols for cytogenetic analysis using G-banding and fluorescence in situ hybridization.
肿瘤细胞系被广泛用作疾病模型，并且越来越多地作为用于确定新癌症基因的基因组资源。细胞遗传学分析仍然是揭示癌症基因组的主要途径。然而，癌细胞系在收获偏好上存在难以解释的差异，因此必须通过反复试验来确定。本文描述了针对550种常用的（主要是人类）癌细胞系凭经验优化的收获方案，以及基于证据的程序，以协助确定未列出细胞系的条件，以及使用G显带和荧光原位杂交进行细胞遗传学分析的辅助方案。
