Borsodi Andrea K, Rusznyák Anna, Molnár Piroska, Vladár Péter, Reskóné Mária N, Tóth Erika M, Sipos Rita, Gedeon Gábor, Márialigeti Károly
Department of Microbiology, Eotvos Loránd University, Budapest, Hungary.
Microb Ecol. 2007 May;53(4):612-20. doi: 10.1007/s00248-006-9133-x. Epub 2007 Apr 4.
In the present study, the species composition and potential metabolic activities of bacterial communities of reed Phragmites australis (Cav.) (Trin. ex Steudel) periphyton from Lake Velencei were studied by cultivation-based and metabolic fingerprinting methods. Serially diluted spring biofilm samples were used to test the community-level physiological profiling (CLPP) using BIOLOG microplates, and for plating onto different media. On the basis of their morphological, biochemical, and physiological test results, 173 strains were clustered by numerical analysis. Representatives of amplified ribosomal DNA restriction analysis (ARDRA) groups were identified by their 16S rDNA sequence comparison. Based on the results of the CLPP investigations, regional differences were detected among the utilized substrate numbers and types, parallel with the increase in incubation time. The phenotypic test results of the strains showed considerable variability with respect to the sampling sites and the media used for cultivation. The most frequently isolated strains were identified as members of genera Agrobacterium, Pseudomonas (P. anguilliseptica, P. marginalis, P. alcaligenes, P. fragi) with aerobic or facultative anaerobic respiratory metabolism, and the species Aeromonas sobria and A. veronii with strong facultative fermentative metabolism. Other strains were identified as Gram-positive Arthrobacter, Bacillus, and Kocuria species. The rarely isolated strains were members of beta-Proteobacteria (Acidovorax, Delftia, Hydrogenophaga, and Rhodoferax), gamma-Proteobacteria (Psychrobacter and Shewanella), low G + C Gram-positives (Brevibacillus, Paenibacillus, and Exiguobacterium) and high G + C Gram-positives (Aureobacterium and Microbacterium).
在本研究中，采用基于培养和代谢指纹图谱的方法，对来自韦伦采湖芦苇（Phragmites australis (Cav.) (Trin. ex Steudel)）周丛生物的细菌群落的物种组成和潜在代谢活性进行了研究。使用连续稀释的春季生物膜样本，通过BIOLOG微孔板测试群落水平的生理特征分析（CLPP），并接种到不同培养基上。根据其形态、生化和生理测试结果，通过数值分析对173株菌株进行了聚类。通过16S rDNA序列比较，确定了扩增核糖体DNA限制性分析（ARDRA）组的代表菌株。基于CLPP研究结果，随着培养时间的增加，在所利用的底物数量和类型上检测到区域差异。菌株的表型测试结果在采样地点和用于培养的培养基方面表现出相当大的变异性。最常分离出的菌株被鉴定为具有需氧或兼性厌氧呼吸代谢的农杆菌属、假单胞菌属（鳗弧菌、边缘假单胞菌、产碱假单胞菌、嗜冷假单胞菌）成员，以及具有强兼性发酵代谢的温和气单胞菌和维氏气单胞菌。其他菌株被鉴定为革兰氏阳性的节杆菌属、芽孢杆菌属和考克氏菌属。很少分离出的菌株是β-变形菌纲（嗜酸菌属、代尔夫特菌属、嗜氢菌属和红环菌属）、γ-变形菌纲（嗜冷杆菌属和希瓦氏菌属）、低G + C革兰氏阳性菌（短短芽孢杆菌、类芽孢杆菌和嗜水气单胞菌）和高G + C革兰氏阳性菌（金色杆菌属和微杆菌属）的成员。
