Borsodi A K, Micsinai A, Rusznyák A, Vladár P, Kovács G, Tóth E M, Márialigeti K
Department of Microbiology, Eötvös Loránd University, Budapest, Hungary.
Microb Ecol. 2005 Jul;50(1):9-18. doi: 10.1007/s00248-004-0063-1. Epub 2005 Aug 18.
Bacterial communities associated with decomposing rhizomes of Phragmites australis were investigated in Lake Ferto (Neusiedlersee, Hungary). Alkaliphilic and alkalitolerant strains were isolated on cellulose-containing alkaline medium spread with dilutions of scrapings taken from the surface of the decaying plant material. Fifty-one strains were grouped by numerical analysis based on physiological tests and BIOLOG sole carbon source utilization data. The strains identified by 16S rDNA sequence comparisons included members of low G+C Gram positives (Marinibacillus marinus, Bacillus cereus, and Exiguobacterium aurantiacum), high G+C Gram positives (Nesterenkonia halobia and Dietzia natronolimnea), alpha-proteobacteria (Pannonibacter phragmitetus), and gamma-proteobacteria (Pseudomonas pseudoalcaligenes and Halomonas venusta). Most of the strains were characterized by aerobic chemoorganotrophic respiratory metabolism and utilized several different carbon sources, although no direct cellulolytic activity was observed. Results of the pH and salt tolerance tests revealed optimuma in most cases at pH 11 and at the presence of 2.5-5% NaCl. These bacteria probably occupy niches in the aerobic, alkaline, water-influenced environments on the decomposing reed surfaces.
在费尔特湖（匈牙利新锡德尔湖）对与芦苇腐烂根茎相关的细菌群落进行了研究。在含有纤维素的碱性培养基上，用从腐烂植物材料表面刮取物的稀释液进行涂布，分离出嗜碱和耐碱菌株。基于生理测试和BIOLOG唯一碳源利用数据，通过数值分析对51株菌株进行了分类。通过16S rDNA序列比较鉴定的菌株包括低G+C革兰氏阳性菌（海栖芽孢杆菌、蜡样芽孢杆菌和橙色栖热菌）、高G+C革兰氏阳性菌（嗜盐涅斯捷连科氏菌和嗜盐迪茨氏菌）、α-变形菌（芦苇泛菌）和γ-变形菌（假产碱假单胞菌和嗜盐盐单胞菌）。大多数菌株的特征是好氧化学有机营养呼吸代谢，并且利用几种不同的碳源，尽管未观察到直接的纤维素分解活性。pH和耐盐性测试结果表明，在大多数情况下，最适pH为11，最适NaCl浓度为2.5-5%。这些细菌可能在腐烂芦苇表面的需氧、碱性、受水影响的环境中占据生态位。
